A mixed-mode liquid chromatography-tandem mass spectrometric method for the determination of cytarabine in mouse plasma

被引：31
作者：
Hsieh, Yunsheng [1 ]
Duncan, Christine J. G. [1 ]
Liu, Ming [1 ]
机构：
[1] Schering Plough Res Inst, Drug Metab & Pharmacokinet Dept, Kenilworth, NJ 07033 USA
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2007年 / 854卷 / 1-2期
关键词：
mixed-mode liquid chromatography; tandem mass spectrometry; cytarabine; matrix ionization suppression;
D O I：
10.1016/j.jchromb.2007.03.034
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A novel mixed-mode high performance liquid chromatographic system (HPLC interfaced with an atmospheric pressure chemical ionization (APCI) source and a tandem mass spectrometer (MS/MS) was developed for the determination of cytarabine (ara-C) in mouse plasma to support pharmacodynamic studies. The mixed-mode reversed-phase ion-exchange chromatography column was adapted for sufficient retention and separation of a small and polar analyte. The impact of the mobile phase composition on both chromatographic separation and the ionization efficiency of the test compound in the positive mode was investigated. The potential of ionization suppression from endogenous biological matrices on the mixed-mode LC-APCI/MS/MS method was evaluated using the post-column infusion technique. Furthermore, the feasibility of using the mixed-mode HPLC-MS/MS method for the determination of the plasma concentrations of cytarabine in mice was demonstrated by comparing those obtained by the ion-pairing HPLC-MS/MS method. (C) 2007 Elsevier B.V. All rights reserved.
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收藏
页码：8 / 12
页数：5
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