5-(3′,4′-Dihydroxyphenyl-γ-valerolactone), a Major Microbial Metabolite of Proanthocyanidin, Attenuates THP-1 Monocyte-Endothelial Adhesion

被引：61

作者：

Lee, Charles C. ^{[1
]}

Kim, Jong Hun ^{[2
]}

Kim, Ji Seung ^{[3
]}

Oh, Yun Sil ^{[3
]}

Han, Seung Min ^{[3
]}

Park, Jung Han Yoon ^{[2
]}

Lee, Ki Won ^{[2
,3
]}

Lee, Chang Yong ^{[1
]}

机构：

[1] Cornell Univ, Dept Food Sci, Ithaca, NY 14853 USA

[2] Seoul Natl Univ, Res Inst Agr & Life Sci, Seoul 08826, South Korea

[3] Seoul Natl Univ, Dept Agr Biotechnol, Major Biomodulat, Seoul 08826, South Korea

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2017年 / 18卷 / 07期

关键词：

5-(3; 4; '-dihydroxyphenyl-gamma-valerolactone); proanthocyanidin microbial metabolite; atherosclerosis prevention; monocyte-endothelial adhesion; RANDOMIZED CONTROLLED-TRIALS; BARK EXTRACT PYCNOGENOL; FLAVANOL-RICH COCOA; IN-VITRO; CARDIOVASCULAR-DISEASE; ANTIOXIDANT ACTIVITY; PROSPECTIVE COHORT; VASCULAR FUNCTION; FLAVONOID INTAKE; RING-FISSION;

D O I：

10.3390/ijms18071363

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Several metabolomics of polymeric flavan-3-ols have reported that proanthocyanidins are extensively metabolized by gut microbiota. 5-(3',4'-dihydroxyphenyl)-gamma-valerolactone (DHPV) has been reported to be the major microbial metabolite of proanthocyanidins. We demonstrated that DHPV has stronger prevention effect on tumor necrosis factor (TNF)-alpha-stimulated adhesion of THP-1 human monocytic cells to human umbilical vein endothelial cells compared to its potential precursors such as procyanidin A1, A2, B1 and B2, (+) catechin, (-)epicatechin and its microbial metabolites such as 3-(3,4-dihydroxyphenyl) propionic acid and 2-(3,4-dihydroxyphenyl) acetic acid. Mechanism study showed that DHPV prevents THP-1 monocyte-endothelial cell adhesion by downregulating TNF-alpha-stimulated expressions of the two biomarkers of atherosclerosis such as vascular cell adhesion molecule-1 and monocyte chemotactic protein-1, activation of nuclear factor kappa B transcription and phosphorylation of I kappa-B kinase and I kappa B alpha. We suggested that DHPV has higher potentiality in prevention of atherosclerosis among the proanthocyanidin metabolites.

引用

页数：12

共 43 条

[1] Procyanidin Dimers Are Metabolized by Human Microbiota with 2-(3,4-Dihydroxyphenyl)acetic Acid and 5-(3,4-Dihydroxyphenyl)-γ-valerolactone as the Major Metabolites [J].