Application of capillary electrophoresis to the analysis of soluble chromatin

被引：0
|
作者：
Asatiani, NV [1 ]
Abuladze, MK [1 ]
Birkaya, BG [1 ]
Sapojnikova, NA [1 ]
Tsibakhashvili, NY [1 ]
Mosulishvili, LM [1 ]
机构：
[1] Georgian Acad Sci, Inst Phys, GE-380077 Tbilisi, Georgia
来源：
BIOMEDICAL CHROMATOGRAPHY | 2000年 / 14卷 / 07期
关键词：
D O I：
10.1002/1099-0801(200011)14:7<489::AID-BMC998>3.0.CO;2-F
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Capillary electrophoresis (CE) has been applied to study DNA-protein complexes using as the test system soluble chromatin from chicken erythrocytes and rapidly proliferated cultured Chinese hamster fibroblast-like cells B11-dii-FAF-28. Separation was performed with home-made CE apparatus, using a regulated high-voltage power supply, UV-detector and fused silica capillaries with inner diameter 75 mum. The heterogeneity of nucleosomal particles with different DNA lengths after micrococcal nuclease digestion was detected. Copyright (C) 2000 John Wiley & Sons, Ltd.
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收藏
页码：489 / 492
页数：4
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