A simple and rapid fluorescence-based immunoassay for the detection of staphylococcal enterotoxin B

被引：23

作者：

Khan, AS ^{[1
]}

Cao, CJ ^{[1
]}

Thompson, RG ^{[1
]}

Valdes, JJ ^{[1
]}

机构：

[1] USA, Edgewood Chem Biol Ctr, AMSSB, RRT, Aberdeen Proving Ground, MD 21010 USA

来源：

MOLECULAR AND CELLULAR PROBES | 2003年 / 17卷 / 2-3期

关键词：

staphylococcal enterotoxin B; immunoassay; detection; biothreat agents; fluorescence;

D O I：

10.1016/S0890-8508(02)00109-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The bioterrorism threat is perceived to be a real challenge to our nation's security. This threat has necessitated the design of better and faster assays for the detection of biothreat agents including staphylococcal enterotoxin B (SEB), a causative agent of food poisoning. This study describes a simple, fast and highly sensitive fluorescence-based immunoassay, in which the antibody is fluorescently-labeled for use in this assay. Use of labeled antibodies resulted in very low level of detection of SEB, 100 pg/well. This method is four times faster than classical and conventional enzyme-linked immunosorbent assay (ELISA). (C) 2003 Elsevier Science Ltd. All rights reserved.

引用

页码：125 / 126

页数：2