Overexpression of WDR79 in non-small cell lung cancer is linked to tumour progression

被引：14

作者：

Sun, Yang ^{[1
]}

Yang, Chao ^{[1
,2
]}

Chen, Jieying ^{[1
]}

Song, Xin ^{[3
]}

Li, Zhen ^{[3
]}

Duan, Minlan ^{[1
]}

Li, Jianglin ^{[1
]}

Hu, Xiaoxiao ^{[1
]}

Wu, Kuangpei ^{[1
]}

Yan, Guobei ^{[1
]}

Yang, Cai ^{[1
]}

Liu, Jing ^{[4
,5
]}

Tan, Weihong ^{[1
]}

Ye, Mao ^{[1
]}

机构：

[1] Hunan Univ, Coll Chem & Chem Engn, Collaborat Innovat Ctr Mol Engn Theranost,Coll Bi, Mol Sci & Biomed Lab,State Key Lab Chemo Biosensi, Changsha 410082, Hunan, Peoples R China

[2] Gannan Normal Univ, Coll Life & Environm Sci, Ganzhou, Jiangxi, Peoples R China

[3] Kunming Med Univ, Tumor Hosp Yunnan Prov, Canc Biotherapy Ctr, Kunming, Yunnan, Peoples R China

[4] Cent S Univ, State Key Lab Med Genet, Changsha, Hunan, Peoples R China

[5] Cent S Univ, Sch Life Sci, Changsha, Hunan, Peoples R China

来源：

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE | 2016年 / 20卷 / 04期

基金：

中国国家自然科学基金;

关键词：

lung cancer; cell cycle; apoptosis; proliferation; WD-REPEAT PROTEIN; OVARIAN-CANCER; CAJAL BODIES; WRAP53; SUSCEPTIBILITY; ASSOCIATION; INDUCTION; THERAPY; GROWTH; TCAB1;

D O I：

10.1111/jcmm.12759

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

WD-repeat protein 79 (WDR79), a member of the WD-repeat protein family, acts as a scaffold protein, participating in telomerase assembly, Cajal body formation and DNA double-strand break repair. Here, we first report that WDR79 is frequently overexpressed in cell lines and tissues derived from non-small cell lung cancer (NSCLC). Knockdown of WDR79 significantly inhibited the proliferation of NSCLC cells in vitro and in vivo by inducing cell cycle arrest and apoptosis. WD-repeat protein 79 -induced cell cycle arrest at the G0/G1 phase was associated with the expression of G0/G1-related cyclins and cyclin-dependent kinase complexes. We also provide evidence that WDR79 knockdown induces apoptosis via a mitochondrial pathway. Collectively, these results suggest that WDR79 is involved in the tumorigenesis of NSCLC and is a potential novel diagnostic marker and therapeutic target for NSCLC.

引用

页码：698 / 709

页数：12

共 31 条

[1] RACK1, A multifaceted scaffolding protein: Structure and function [J].

Adams, David R. ;

Ron, Dorit ;

Kiely, Patrick A. .

CELL COMMUNICATION AND SIGNALING, 2011, 9

[2] Death receptors: Signaling and modulation [J].

Ashkenazi, A ;

Dixit, VM .

SCIENCE, 1998, 281 (5381) :1305-1308

[3] FLASH Knockdown Sensitizes Cells To Fas-Mediated Apoptosis via Down-Regulation of the Anti-Apoptotic Proteins, MCL-1 and Cflip Short [J].

Chen, Song ;

Evans, Hedeel Guy ;

Evans, David R. .

PLOS ONE, 2012, 7 (03)

[4] Wrap53, a novel regulator of p53 [J].

Farnebo, Marianne .

CELL CYCLE, 2009, 8 (15) :2343-2346

[5] Common genetic variation in TP53 and its flanking genes, WDR79 and ATP1B2, and susceptibility to breast cancer [J].

Garcia-Closas, Montserrat ;

Kristensen, Vessela ;

Langerod, Anita ;

Qi, Ying ;

Yeager, Meredith ;

Burdett, Laurie ;

Welch, Robert ;

Lissowska, Jolanta ;

Peplonska, Beata ;

Brinton, Louise ;

Gerhard, Daniela S. ;

Gram, Inger Torhild ;

Perou, Charles M. ;

Borresen-Dale, Anne-Lise ;

Chanock, Stephen .

INTERNATIONAL JOURNAL OF CANCER, 2007, 121 (11) :2532-2538

[6] Oncogenic function of a novel WD-domain protein, STRAP, in human carcinogenesis [J].

Haider, Sunil K. ;

Anumanthan, Govindaraj ;

Maddula, Ramakoti ;

Mann, Jason ;

Chytil, Anna ;

Gonzalez, Adriana L. ;

Washington, M. Key ;

Moses, Harold L. ;

Beauchamp, R. Daniel ;

Datta, Pran K. .

CANCER RESEARCH, 2006, 66 (12) :6156-6166

[7] On the road with WRAP53β: guardian of Cajal bodies and genome integrity [J].

Henriksson, Sofia ;

Farnebo, Marianne .

FRONTIERS IN GENETICS, 2015, 6

[8] The scaffold protein WRAP53β orchestrates the ubiquitin response critical for DNA double-strand break repair [J].

Henriksson, Sofia ;

Rassoolzadeh, Hanif ;

Hedstrom, Elisabeth ;

Coucoravas, Christos ;

Julner, Alexander ;

Goldstein, Michael ;

Imreh, Gabriela ;

Zhivotovsky, Boris ;

Kastan, Michael B. ;

Helleday, Thomas ;

Farnebo, Marianne .

GENES & DEVELOPMENT, 2014, 28 (24) :2726-2738

[9] TELOMERASE ACTIVITY IN SMALL-CELL AND NON-SMALL-CELL LUNG CANCERS [J].

HIYAMA, K ;

HIYAMA, E ;

ISHIOKA, S ;

YAMAKIDO, M ;

INAI, K ;

GAZDAR, AF ;

PIATYSZEK, MA ;

SHAY, JW .

JOURNAL OF THE NATIONAL CANCER INSTITUTE, 1995, 87 (12) :895-902

[10] Endonuclein is a cell cycle regulated WD-repeat protein that is up-regulated in adenocarcinoma of the pancreas [J].

Honore, B ;

Baandrup, U ;

Nielsen, S ;

Vorum, H .

ONCOGENE, 2002, 21 (07) :1123-1129

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