In the current pandemic of coronavirus disease (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the co-circulation of SARS-CoV-2 and other respiratory viruses during the upcoming fall and winter seasons may present an unprecedented burden of respiratory disease in the population. Important respiratory viruses that will need to be closely monitored during this time include SARS-CoV-2, influenza A and influenza B. The epidemiology of these viruses is very similar in terms of susceptible populations, mode of transmission, and the clinical syndromes, thus the etiological agent will be difficult to differentiate without target specific assays. The availability of a sensitive and specific multiplex assay that can simultaneously detect all these targets will be valuable. Here we report the validation of a real-time reverse transciptase-PCR assay for the simultaneous detection of SARS-CoV-2, influenza A and influenza B. This multiplex assay is comparable to its singleplex counterparts with a limit-of-detection being less than 5 copies/reaction, 100 % specificity, over seven logs of dynamic range, less than 1 % coefficientof variation showing high precision, and equivalent accuracy using patient samples. It also offers the added benefits of savings in reagents and technologist time while improving testing efficiency and turn-around-times in order to respond effectively to the ongoing pandemic.
机构:
Icahn Sch Med Mt Sinai, Dept Microbiol, New York, NY 10029 USAIcahn Sch Med Mt Sinai, Dept Microbiol, New York, NY 10029 USA
Krammer, Florian
Smith, Gavin J. D.
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Duke NUS Med Sch, Singapore, Singapore
Duke Univ, Duke Global Hlth Inst, Durham, NC USAIcahn Sch Med Mt Sinai, Dept Microbiol, New York, NY 10029 USA
Smith, Gavin J. D.
Fouchier, Ron A. M.
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Erasmus MC, Dept Virosci, Rotterdam, NetherlandsIcahn Sch Med Mt Sinai, Dept Microbiol, New York, NY 10029 USA
Fouchier, Ron A. M.
Peiris, Malik
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机构:
Univ Hong Kong, Sch Publ Hlth, Li Ka Shing Fac Med, WHO Collaborating Ctr Infect Dis Epidemiol & Cont, Hong Kong, Hong Kong, Peoples R China
Univ Hong Kong, Li Ka Shing Fac Med, Ctr Influenza Res, Hong Kong, Hong Kong, Peoples R ChinaIcahn Sch Med Mt Sinai, Dept Microbiol, New York, NY 10029 USA
Peiris, Malik
Kedzierska, Katherine
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h-index: 0
机构:
Univ Melbourne, Peter Doherty Inst Infect & Immun, Dept Microbiol & Immunol, Melbourne, Vic, AustraliaIcahn Sch Med Mt Sinai, Dept Microbiol, New York, NY 10029 USA
Kedzierska, Katherine
Doherty, Peter C.
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h-index: 0
机构:
Univ Melbourne, Peter Doherty Inst Infect & Immun, Dept Microbiol & Immunol, Melbourne, Vic, Australia
St Jude Childrens Res Hosp, Dept Immunol, 332 N Lauderdale St, Memphis, TN 38105 USAIcahn Sch Med Mt Sinai, Dept Microbiol, New York, NY 10029 USA
Doherty, Peter C.
Palese, Peter
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h-index: 0
机构:
Icahn Sch Med Mt Sinai, Dept Microbiol, New York, NY 10029 USA
Icahn Sch Med Mt Sinai, Dept Med, Div Infect Dis, New York, NY 10029 USAIcahn Sch Med Mt Sinai, Dept Microbiol, New York, NY 10029 USA
Palese, Peter
Shaw, Megan L.
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h-index: 0
机构:
Icahn Sch Med Mt Sinai, Dept Microbiol, New York, NY 10029 USAIcahn Sch Med Mt Sinai, Dept Microbiol, New York, NY 10029 USA
Shaw, Megan L.
Treanor, John
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h-index: 0
机构:
Univ Rochester, Sch Med & Dent, Dept Med, Div Infect Dis, Rochester, NY 14642 USAIcahn Sch Med Mt Sinai, Dept Microbiol, New York, NY 10029 USA
Treanor, John
Webster, Robert G.
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h-index: 0
机构:
St Jude Childrens Res Hosp, Dept Infect Dis, 332 N Lauderdale St, Memphis, TN 38105 USAIcahn Sch Med Mt Sinai, Dept Microbiol, New York, NY 10029 USA
Webster, Robert G.
Garcia-Sastre, Adolfo
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机构:
Icahn Sch Med Mt Sinai, Dept Microbiol, New York, NY 10029 USA
Icahn Sch Med Mt Sinai, Dept Med, Div Infect Dis, New York, NY 10029 USA
Icahn Sch Med Mt Sinai, Global Hlth & Emerging Pathogens Inst, New York, NY 10029 USAIcahn Sch Med Mt Sinai, Dept Microbiol, New York, NY 10029 USA
机构:
Univ Med Ctr, Julius Ctr Hlth Sci & Primary Care, Utrecht, NetherlandsUniv Med Ctr, Julius Ctr Hlth Sci & Primary Care, Utrecht, Netherlands
Kretzschmar, Mirjam E.
Rozhnova, Ganna
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h-index: 0
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Univ Med Ctr, Julius Ctr Hlth Sci & Primary Care, Utrecht, Netherlands
Univ Lisbon, Fac Ciencias, BioISI Biosyst & Integrat Sci Inst, Lisbon, PortugalUniv Med Ctr, Julius Ctr Hlth Sci & Primary Care, Utrecht, Netherlands
Rozhnova, Ganna
Bootsma, Martin C. J.
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h-index: 0
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Univ Med Ctr, Julius Ctr Hlth Sci & Primary Care, Utrecht, Netherlands
Univ Utrecht, Math Inst, Utrecht, NetherlandsUniv Med Ctr, Julius Ctr Hlth Sci & Primary Care, Utrecht, Netherlands
Bootsma, Martin C. J.
van Boven, Michiel
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Univ Med Ctr, Julius Ctr Hlth Sci & Primary Care, Utrecht, NetherlandsUniv Med Ctr, Julius Ctr Hlth Sci & Primary Care, Utrecht, Netherlands
van Boven, Michiel
van de Wijgert, Janneke H. H. M.
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Univ Med Ctr, Julius Ctr Hlth Sci & Primary Care, Utrecht, Netherlands
Univ Liverpool, Inst Infect & Global Hlth, Liverpool, Merseyside, EnglandUniv Med Ctr, Julius Ctr Hlth Sci & Primary Care, Utrecht, Netherlands
van de Wijgert, Janneke H. H. M.
Bonten, Marc J. M.
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机构:
Univ Med Ctr, Julius Ctr Hlth Sci & Primary Care, Utrecht, Netherlands
Univ Med Ctr, Dept Med Microbiol, Utrecht, NetherlandsUniv Med Ctr, Julius Ctr Hlth Sci & Primary Care, Utrecht, Netherlands
机构:
London Sch Hyg & Trop Med, Ctr Math Modelling Infect Dis, London WC1E 7HT, EnglandLondon Sch Hyg & Trop Med, Ctr Math Modelling Infect Dis, London WC1E 7HT, England
Kucharski, Adam J.
Klepac, Petra
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h-index: 0
机构:
London Sch Hyg & Trop Med, Ctr Math Modelling Infect Dis, London WC1E 7HT, England
Univ Cambridge, Dept Appl Math & Theoret Phys, Cambridge, EnglandLondon Sch Hyg & Trop Med, Ctr Math Modelling Infect Dis, London WC1E 7HT, England
Klepac, Petra
Conlan, Andrew J. K.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Cambridge, Dept Vet Med, Cambridge, EnglandLondon Sch Hyg & Trop Med, Ctr Math Modelling Infect Dis, London WC1E 7HT, England
Conlan, Andrew J. K.
Kissler, Stephen M.
论文数: 0引用数: 0
h-index: 0
机构:
Harvard TH Chan Sch Publ Hlth, Dept Immunol & Infect Dis, Boston, MA USALondon Sch Hyg & Trop Med, Ctr Math Modelling Infect Dis, London WC1E 7HT, England
Kissler, Stephen M.
Tang, Maria L.
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h-index: 0
机构:
Univ Cambridge, Dept Appl Math & Theoret Phys, Cambridge, EnglandLondon Sch Hyg & Trop Med, Ctr Math Modelling Infect Dis, London WC1E 7HT, England
Tang, Maria L.
Fry, Hannah
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h-index: 0
机构:
UCL, Ctr Adv Spatial Anal, London, EnglandLondon Sch Hyg & Trop Med, Ctr Math Modelling Infect Dis, London WC1E 7HT, England
Fry, Hannah
Gog, Julia R.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Cambridge, Dept Appl Math & Theoret Phys, Cambridge, EnglandLondon Sch Hyg & Trop Med, Ctr Math Modelling Infect Dis, London WC1E 7HT, England
Gog, Julia R.
Edmunds, W. John
论文数: 0引用数: 0
h-index: 0
机构:
London Sch Hyg & Trop Med, Ctr Math Modelling Infect Dis, London WC1E 7HT, EnglandLondon Sch Hyg & Trop Med, Ctr Math Modelling Infect Dis, London WC1E 7HT, England
机构:
Icahn Sch Med Mt Sinai, Dept Microbiol, New York, NY 10029 USAIcahn Sch Med Mt Sinai, Dept Microbiol, New York, NY 10029 USA
Krammer, Florian
Smith, Gavin J. D.
论文数: 0引用数: 0
h-index: 0
机构:
Duke NUS Med Sch, Singapore, Singapore
Duke Univ, Duke Global Hlth Inst, Durham, NC USAIcahn Sch Med Mt Sinai, Dept Microbiol, New York, NY 10029 USA
Smith, Gavin J. D.
Fouchier, Ron A. M.
论文数: 0引用数: 0
h-index: 0
机构:
Erasmus MC, Dept Virosci, Rotterdam, NetherlandsIcahn Sch Med Mt Sinai, Dept Microbiol, New York, NY 10029 USA
Fouchier, Ron A. M.
Peiris, Malik
论文数: 0引用数: 0
h-index: 0
机构:
Univ Hong Kong, Sch Publ Hlth, Li Ka Shing Fac Med, WHO Collaborating Ctr Infect Dis Epidemiol & Cont, Hong Kong, Hong Kong, Peoples R China
Univ Hong Kong, Li Ka Shing Fac Med, Ctr Influenza Res, Hong Kong, Hong Kong, Peoples R ChinaIcahn Sch Med Mt Sinai, Dept Microbiol, New York, NY 10029 USA
Peiris, Malik
Kedzierska, Katherine
论文数: 0引用数: 0
h-index: 0
机构:
Univ Melbourne, Peter Doherty Inst Infect & Immun, Dept Microbiol & Immunol, Melbourne, Vic, AustraliaIcahn Sch Med Mt Sinai, Dept Microbiol, New York, NY 10029 USA
Kedzierska, Katherine
Doherty, Peter C.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Melbourne, Peter Doherty Inst Infect & Immun, Dept Microbiol & Immunol, Melbourne, Vic, Australia
St Jude Childrens Res Hosp, Dept Immunol, 332 N Lauderdale St, Memphis, TN 38105 USAIcahn Sch Med Mt Sinai, Dept Microbiol, New York, NY 10029 USA
Doherty, Peter C.
Palese, Peter
论文数: 0引用数: 0
h-index: 0
机构:
Icahn Sch Med Mt Sinai, Dept Microbiol, New York, NY 10029 USA
Icahn Sch Med Mt Sinai, Dept Med, Div Infect Dis, New York, NY 10029 USAIcahn Sch Med Mt Sinai, Dept Microbiol, New York, NY 10029 USA
Palese, Peter
Shaw, Megan L.
论文数: 0引用数: 0
h-index: 0
机构:
Icahn Sch Med Mt Sinai, Dept Microbiol, New York, NY 10029 USAIcahn Sch Med Mt Sinai, Dept Microbiol, New York, NY 10029 USA
Shaw, Megan L.
Treanor, John
论文数: 0引用数: 0
h-index: 0
机构:
Univ Rochester, Sch Med & Dent, Dept Med, Div Infect Dis, Rochester, NY 14642 USAIcahn Sch Med Mt Sinai, Dept Microbiol, New York, NY 10029 USA
Treanor, John
Webster, Robert G.
论文数: 0引用数: 0
h-index: 0
机构:
St Jude Childrens Res Hosp, Dept Infect Dis, 332 N Lauderdale St, Memphis, TN 38105 USAIcahn Sch Med Mt Sinai, Dept Microbiol, New York, NY 10029 USA
Webster, Robert G.
Garcia-Sastre, Adolfo
论文数: 0引用数: 0
h-index: 0
机构:
Icahn Sch Med Mt Sinai, Dept Microbiol, New York, NY 10029 USA
Icahn Sch Med Mt Sinai, Dept Med, Div Infect Dis, New York, NY 10029 USA
Icahn Sch Med Mt Sinai, Global Hlth & Emerging Pathogens Inst, New York, NY 10029 USAIcahn Sch Med Mt Sinai, Dept Microbiol, New York, NY 10029 USA
机构:
Univ Med Ctr, Julius Ctr Hlth Sci & Primary Care, Utrecht, NetherlandsUniv Med Ctr, Julius Ctr Hlth Sci & Primary Care, Utrecht, Netherlands
Kretzschmar, Mirjam E.
Rozhnova, Ganna
论文数: 0引用数: 0
h-index: 0
机构:
Univ Med Ctr, Julius Ctr Hlth Sci & Primary Care, Utrecht, Netherlands
Univ Lisbon, Fac Ciencias, BioISI Biosyst & Integrat Sci Inst, Lisbon, PortugalUniv Med Ctr, Julius Ctr Hlth Sci & Primary Care, Utrecht, Netherlands
Rozhnova, Ganna
Bootsma, Martin C. J.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Med Ctr, Julius Ctr Hlth Sci & Primary Care, Utrecht, Netherlands
Univ Utrecht, Math Inst, Utrecht, NetherlandsUniv Med Ctr, Julius Ctr Hlth Sci & Primary Care, Utrecht, Netherlands
Bootsma, Martin C. J.
van Boven, Michiel
论文数: 0引用数: 0
h-index: 0
机构:
Univ Med Ctr, Julius Ctr Hlth Sci & Primary Care, Utrecht, NetherlandsUniv Med Ctr, Julius Ctr Hlth Sci & Primary Care, Utrecht, Netherlands
van Boven, Michiel
van de Wijgert, Janneke H. H. M.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Med Ctr, Julius Ctr Hlth Sci & Primary Care, Utrecht, Netherlands
Univ Liverpool, Inst Infect & Global Hlth, Liverpool, Merseyside, EnglandUniv Med Ctr, Julius Ctr Hlth Sci & Primary Care, Utrecht, Netherlands
van de Wijgert, Janneke H. H. M.
Bonten, Marc J. M.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Med Ctr, Julius Ctr Hlth Sci & Primary Care, Utrecht, Netherlands
Univ Med Ctr, Dept Med Microbiol, Utrecht, NetherlandsUniv Med Ctr, Julius Ctr Hlth Sci & Primary Care, Utrecht, Netherlands
机构:
London Sch Hyg & Trop Med, Ctr Math Modelling Infect Dis, London WC1E 7HT, EnglandLondon Sch Hyg & Trop Med, Ctr Math Modelling Infect Dis, London WC1E 7HT, England
Kucharski, Adam J.
Klepac, Petra
论文数: 0引用数: 0
h-index: 0
机构:
London Sch Hyg & Trop Med, Ctr Math Modelling Infect Dis, London WC1E 7HT, England
Univ Cambridge, Dept Appl Math & Theoret Phys, Cambridge, EnglandLondon Sch Hyg & Trop Med, Ctr Math Modelling Infect Dis, London WC1E 7HT, England
Klepac, Petra
Conlan, Andrew J. K.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Cambridge, Dept Vet Med, Cambridge, EnglandLondon Sch Hyg & Trop Med, Ctr Math Modelling Infect Dis, London WC1E 7HT, England
Conlan, Andrew J. K.
Kissler, Stephen M.
论文数: 0引用数: 0
h-index: 0
机构:
Harvard TH Chan Sch Publ Hlth, Dept Immunol & Infect Dis, Boston, MA USALondon Sch Hyg & Trop Med, Ctr Math Modelling Infect Dis, London WC1E 7HT, England
Kissler, Stephen M.
Tang, Maria L.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Cambridge, Dept Appl Math & Theoret Phys, Cambridge, EnglandLondon Sch Hyg & Trop Med, Ctr Math Modelling Infect Dis, London WC1E 7HT, England
Tang, Maria L.
Fry, Hannah
论文数: 0引用数: 0
h-index: 0
机构:
UCL, Ctr Adv Spatial Anal, London, EnglandLondon Sch Hyg & Trop Med, Ctr Math Modelling Infect Dis, London WC1E 7HT, England
Fry, Hannah
Gog, Julia R.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Cambridge, Dept Appl Math & Theoret Phys, Cambridge, EnglandLondon Sch Hyg & Trop Med, Ctr Math Modelling Infect Dis, London WC1E 7HT, England
Gog, Julia R.
Edmunds, W. John
论文数: 0引用数: 0
h-index: 0
机构:
London Sch Hyg & Trop Med, Ctr Math Modelling Infect Dis, London WC1E 7HT, EnglandLondon Sch Hyg & Trop Med, Ctr Math Modelling Infect Dis, London WC1E 7HT, England