Determination of kanamycin sulfate by liquid chromatography with pulsed amperometric detection

A liquid chromatography (LC) with pulsed amperometric detection (PAD) method for the determination of kanamycin on a gold electode was developed. The mobile phase consisted of an aqueous solution containing 0.033 mol/L oxalic acid, 0.012 mol/L heptafluorobutyric acid and 105 mL/L acetonitrile with pH 3.40 adjusting by dilute NaOH solution. In comparison with the methods previously published, the total analysis time was short not more than 35 min, and the method allows not only a better separation of kanamycin A and kanamycin B, but also the separation of several other minor components I although most of which were not identified. The effects of the different chromatographic parameters on the separation were also investigated. The method was simple, accurate and wihtout requirement for derivatization steps.