iTRAQ-based proteomic analysis of Paracoccidioides brasiliensis in response to hypoxia

被引:7
|
作者
Oliveira, Lucas Nojosa [1 ]
Lima, Patricia de Sousa [1 ]
Araujo, Danielle Silva [1 ]
Portis, Igor Godinho [1 ]
Moreira dos Santos Junior, Agenor de Castro [2 ]
Guedes Coelho, Alexandre Siqueira [3 ]
de Sousa, Marcelo Valle [2 ]
Ornelas Ricart, Carlos Andre [2 ]
Fontes, Wagner [2 ]
de Almeida Soares, Celia Maria [1 ]
机构
[1] Univ Fed Goias, Inst Ciencias Biol, Lab Biol Mol, ICB 2, Campus 2, BR-74001970 Goiania, Go, Brazil
[2] Univ Brasilia, Inst Biol, Dept Biol Celular, Campus Darcy Ribeiro, BR-70910900 Brasilia, DF, Brazil
[3] Univ Fed Goias, Lab Genet & Genom Plantas, Escola Agron, Goiania, Go, Brazil
关键词
Low oxygen; Pb18; Isobaric tag proteomics; PHOSPHOENOLPYRUVATE CARBOXYKINASE; ASPERGILLUS-NIDULANS; CANDIDA-ALBICANS; SACCHAROMYCES-CEREVISIAE; MUTANTS DEFICIENT; STEROL SYNTHESIS; GENE-EXPRESSION; PROTEIN; FORMAMIDASE; ALPHA-(1,3)-GLUCAN;
D O I
10.1016/j.micres.2021.126730
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Aerobic organisms require oxygen for energy. In the course of the infection, adaptation to hypoxia is crucial for survival of human pathogenic fungi. Members of the Paracoccidioides complex face decreased oxygen tensions during the life cycle stages. In Paracoccidioides brasiliensis proteomic responses to hypoxia have not been investigated and the regulation of the adaptive process is still unknown, and this approach allowed the identification of 216 differentially expressed proteins in hypoxia using iTRAQ-labelling. Data suggest that P. brasiliensis reprograms its metabolism when submitted to hypoxia. The fungus reduces its basal metabolism and general transport proteins. Energy and general metabolism were more representative and up regulated. Glucose is apparently directed towards glycolysis or the production of cell wall polymers. Plasma membrane/cell wall are modulated by increasing ergosterol and glucan, respectively. In addition, molecules such as ethanol and acetate are produced by this fungus indicating that alternative carbon sources probably are activated to obtain energy. Also, detoxification mechanisms are activated. The results were compared with label free proteomics data from Paracoccidioides lutzii. Biochemical pathways involved with acetyl-CoA, pyruvate and ergosterol synthesis were up-regulated in both fungi. On the other hand, proteins from TCA, transcription, protein fate/degradation, cellular transport, signal transduction and cell defense/virulence processes presented different profiles between species. Particularly, proteins related to methylcitrate cycle and those involved with acetate and ethanol synthesis were increased in P. brasiliensis proteome, whereas GABA shunt were accumulated only in P. lutzii. The results emphasize metabolic adaptation processes for distinct Paracoccidioides species.
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页数:13
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