Deoxynivalenol Induces Intestinal Damage and Inflammatory Response through the Nuclear Factor-κB Signaling Pathway in Piglets

被引：33

作者：

Wang, Xi-Chun ^{[1
]}

Zhang, Ya-Fei ^{[1
]}

Cao, Li ^{[1
]}

Zhu, Lei ^{[1
]}

Huang, Ying-Ying ^{[1
]}

Chen, Xiao-Fang ^{[1
]}

Chu, Xiao-Yan ^{[1
]}

Zhu, Dian-Feng ^{[1
]}

Rahman, Sajid Ur ^{[1
]}

Feng, Shi-Bin ^{[1
]}

Li, Yu ^{[1
]}

Wu, Jin-Jie ^{[1
]}

机构：

[1] Anhui Agr Univ, Coll Anim Sci & Technol, 130 West Changjiang Rd, Hefei 230036, Anhui, Peoples R China

来源：

TOXINS | 2019年 / 11卷 / 11期

基金：

中国国家自然科学基金;

关键词：

deoxynivalenol; intestinal mucosal injury; inflammatory cytokine; nuclear factor-kappa B signaling; piglets; APOPTOSIS; MYCOTOXINS; CELLS; TNF; POULTRY; IMPACTS;

D O I：

10.3390/toxins11110663

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Deoxynivalenol (DON) is highly toxic to animals and humans, but pigs are most sensitive to it. The porcine mucosal injury related mechanism of DON is not yet fully clarified. Here, we investigated DON-induced injury in the intestinal tissues of piglet. Thirty weanling piglets [(Duroc x Landrace) x Yorkshire] were randomly divided into three groups according to single factor experimental design (10 piglets each group). Piglets were fed a basal diet in the control group, while low and high dose groups were fed a DON diet (1300 and 2200 mu g/kg, respectively) for 60 days. Scanning electron microscopy results indicated that the ultrastructure of intestinal epithelial cells in the DON-treated group was damaged. The distribution and optical density (OD) values of zonula occludens 1 (ZO-1) protein in the intestinal tissues of DON-treated groups were decreased. At higher DON dosage, interleukin (IL)-1 beta, IL-6, and tumor necrosis factor-alpha mRNA levels were elevated in the intestinal tissues. The mRNA and protein levels of NF-kappa B p65, I kappa B-alpha, IKK alpha/beta, iNOS, and COX-2 in the small intestinal mucosa were abnormally altered with an increase in DON concentration. These results indicate that DON can persuade intestinal damage and inflammatory responses in piglets via the nuclear factor-kappa B signaling pathway.

引用

页数：14

共 44 条

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