Quantitation of the Ribosomal Protein Autoregulatory Network Using Mass Spectrometry

被引：15
作者：
Sykes, Michael T.
Sperling, Edit
Chen, Stephen S.
Williamson, James R. [1 ]
机构：
[1] Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA
来源：
ANALYTICAL CHEMISTRY | 2010年 / 82卷 / 12期
基金：
美国国家卫生研究院;
关键词：
ESCHERICHIA-COLI RIBOSOMES; ABSOLUTE QUANTIFICATION; PROTEOMICS; EXPRESSION; PEPTIDES; KINETICS; SILAC;
D O I：
10.1021/ac9028664
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Relative levels of ribosomal proteins were quantified in crude cell lysates using mass spectrometry. A method for quantifying cellular protein levels using macromolecular standards is presented that does not require complex sample separation, identification of high-responding peptides, affinity purification, or postgrowth modifications. Perturbations in ribosomal protein levels by overexpression of individual proteins correlate to known autoregulatory mechanisms and extend the network of ribosomal protein regulation.
引用
收藏
页码：5038 / 5045
页数：8
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