Amylase and acid protease production by solid state fermentation using Aspergillus niger from mangrove swamp

被引：0
作者：
Bhatnagar, D. [1 ]
Joseph, Imelda [2 ]
Raj, R. Paul [2 ]
机构：
[1] Cent Inst Freshwater Aquaculture, Bhubaneswar 751002, Orissa, India
[2] Cent Marine Fisheries Res Inst, Kochi 682018, Kerala, India
来源：
INDIAN JOURNAL OF FISHERIES | 2010年 / 57卷 / 01期
关键词：
Acid protease; Amylases; Aspergillus niger; Mangrove; Solid state fermentation; Wheat bran; GLUCOAMYLASE PRODUCTION; STRAIN; OPTIMIZATION; STARCH; ORYZAE; ALPHA; BRAN;
D O I：
暂无
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
Production of amylase and acid protease by solid state fermentation employing Aspergillus niger strain S(1)4, a mangrove isolate was evaluated. Wheat bran containing 60% moisture was used for fermentation with two inoculum sizes of 2x10(6) and 40x10(6) spores 10 g substrate(-1). The maximum amylase activity of 48.13 Ugdfs(-1) was recorded after an incubation period of 4 days at temperature 30 degrees C and pH 4.8 for the inoculum size 40x10(6) spores 10 g substrate(-1). The concentration of reducing sugar showed a significant negative correlation (-0.496, p<0.05) with amylase production. The peak acid protease activity was observed on day 6 (54.89 Ugdfs(-1)) for 2x10(6) spores 10g substrate(-1) and on day 4 for 40x10(6) spores 10g substrate(-1) under standard assay conditions of 30 degrees C and pH 2.7. The concentration of protein in the substrate was significantly (R=0.778, p<0.05) correlated with the acid protease production.
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页码：45 / 51
页数：7
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