Protective effects of Lactobacillus plantarum on epithelial barrier disruption caused by enterotoxigenic Escherichia coli in intestinal porcine epithelial cells

被引:108
作者
Wu, Yunpeng [1 ,2 ]
Zhu, Cui [2 ]
Chen, Zhuang [2 ]
Chen, Zhongjian [2 ]
Zhang, Weina [2 ]
Ma, Xianyong [3 ]
Wang, Li [3 ]
Yang, Xuefen [3 ]
Jiang, Zongyong [2 ,3 ]
机构
[1] South China Agr Univ, Coll Anim Sci, Guangzhou 510642, Guangdong, Peoples R China
[2] Guangdong Acad Agr Sci, Agrobiol Gene Res Ctr, 29 Jinying Rd, Guangzhou 510640, Guangdong, Peoples R China
[3] Guangdong Acad Agr Sci, Inst Anim Sci, Guangdong Publ Lab Anim Breeding & Nutr, Minist Agr,Key Lab Anim Nutr & Feed Sci South Chi, Guangzhou 510640, Guangdong, Peoples R China
基金
中国国家自然科学基金;
关键词
Lactobacilli plantarum; Tight junction; IPEC-J2; Enterotoxigenic Escherichia coli K88; Epithelial barrier; Probiotic; TIGHT JUNCTION PROTEINS; IN-VITRO; EXPRESSION; PIGLETS; LINE; REUTERI; INTERLEUKIN-8; PERMEABILITY; PATHOGENESIS; ENTEROCYTES;
D O I
10.1016/j.vetimm.2016.03.005
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Tight junctions (TJs) play an important role in maintaining the mucosal barrier function and gastrointestinal health of animals. Lactobacillus plantarum (L. plantarum) was reported to protect the intestinal barrier function of early-weaned piglets against enterotoxigenic Escherichia coli (ETEC) K88 challenge; however, the underlying cellular mechanism of this protection was unclear. Here, an established intestinal porcine epithelia cell (IPEC-J2) model was used to investigate the protective effects and related mechanisms of L. plantarum on epithelial barrier damages induced by ETEC K88. Epithelial permeability, expression of inflammatory cytokines, and abundance of TJ proteins, were determined. Pre-treatment with L. plantarum for 611 prevented the reduction in transepithelial electrical resistance (TEER) (P<0.05), inhibited the increased transcript abundances of interleukin-8 (IL-8) and tumor necrosis factor (TNF-alpha) (P < 0.05), decreased expression of claudin-1, occludin and zonula occludens (ZO-1)(P<0.05) and protein expression of occludin (P<0.05) of IPEC-J2 cells caused by ETEC K88. Moreover, the mRNA expression of negative regulators of toll-like receptors (TLRs) [single Ig IL-1-related receptor (SIGIRR), B-cell CLL/lymphoma 3 (BM), and mitogen-activated protein kinase phosphatase-1 (MKP-1)] in IPEC-J2 cells pre-treated with L. plantarum were higher (P<0.05) compared with those in cells just exposed to K88. Furthermore, L. plantarum was shown to regulate proteins of nuclear factor kappa B (NF-kappa B) and mitogen-activated protein kinase (MAPK) signaling pathways. These results indicated that L plantarum may improve epithelial barrier function by maintenance of TEER, inhibiting the reduction of TJ proteins, and reducing the expression of proinflammatory cytokines induced by ETEC K88, possibly through modulation of TLRs, NF-kappa B and MAPK pathways. (C) 2016 Published by Elsevier B.V.
引用
收藏
页码:55 / 63
页数:9
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