Enhancement of cytotoxicity against Vero E6 cells persistently infected with SARS-CoV by Mycoplasma fermentans

被引：1
作者：
Mizutani, T.
Fukushi, S.
Kenri, T.
Sasaki, Y.
Ishii, K.
Endoh, D.
Zamoto, A.
Saijo, M.
Kurane, I.
Morikawa, S.
机构：
[1] Natl Inst Infect Dis, Dept Virol 1, Tokyo 2080011, Japan
[2] Natl Inst Infect Dis, Dept Bacteriol 2, Tokyo 2080011, Japan
[3] Natl Inst Infect Dis, Dept Virol 2, Tokyo 2080011, Japan
[4] Natl Inst Infect Dis, Div Expt Anim Res, Tokyo 2080011, Japan
[5] Rakuno Gakuen Univ, Sch Vet Med, Lab Vet Radiol, Ebetsu, Hokkaido 069, Japan
来源：
ARCHIVES OF VIROLOGY | 2007年 / 152卷 / 05期
关键词：
D O I：
10.1007/s00705-006-0924-7
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We previously reported that cells with persistent severe acute respiratory syndrome coronavirus (SARS-CoV) infection were established after apoptotic events. In the present study, we investigated the cytopathic effects of dual infection with SARS-CoV and Mycoplasma fermentans on Vero E6 cells. Dual infection completely killed cells and prevented the establishment of persistent SARS-CoV infection. M. fermentans induced inhibition of cell proliferation, but the cells remained alive. Apoptosis was induced easily in M. fermentans-infected cells, indicating that they were primed for apoptosis. These results indicated that M. fermentans enhances apoptosis in surviving cells that have escaped from SARS-CoV-induced apoptosis.
引用
收藏
页码：1019 / 1025
页数：7
相关论文
共 26 条
[21]   Characterization of a novel coronavirus associated with severe acute respiratory syndrome [J].
Rota, PA ;
Oberste, MS ;
Monroe, SS ;
Nix, WA ;
Campagnoli, R ;
Icenogle, JP ;
Peñaranda, S ;
Bankamp, B ;
Maher, K ;
Chen, MH ;
Tong, SX ;
Tamin, A ;
Lowe, L ;
Frace, M ;
DeRisi, JL ;
Chen, Q ;
Wang, D ;
Erdman, DD ;
Peret, TCT ;
Burns, C ;
Ksiazek, TG ;
Rollin, PE ;
Sanchez, A ;
Liffick, S ;
Holloway, B ;
Limor, J ;
McCaustland, K ;
Olsen-Rasmussen, M ;
Fouchier, R ;
Günther, S ;
Osterhaus, ADME ;
Drosten, C ;
Pallansch, MA ;
Anderson, LJ ;
Bellini, WJ .
SCIENCE, 2003, 300 (5624) :1394-1399
[22]   MYCOPLASMAS STIMULATE REPLICATION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 THROUGH SELECTIVE ACTIVATION OF CD4+ T-LYMPHOCYTES [J].
SASAKI, Y ;
HONDA, M ;
MAKINO, M ;
SASAKI, T .
AIDS RESEARCH AND HUMAN RETROVIRUSES, 1993, 9 (08) :775-780
[23]   AIDS-ASSOCIATED MYCOPLASMAS POSSESS PHOSPHOLIPASES-C IN THE MEMBRANE [J].
SHIBATA, K ;
SASAKI, T ;
WATANABE, T .
INFECTION AND IMMUNITY, 1995, 63 (10) :4174-4177
[24]   Lipid-associated membrane proteins of Mycoplasma fermentans and M-penetrans activate human immunodeficiency virus long-terminal repeats through Toll-like receptors [J].
Shimizu, T ;
Kida, Y ;
Kuwano, K .
IMMUNOLOGY, 2004, 113 (01) :121-129
[25]   Establishment of Vero E6 cell clones persistently infected with severe acute respiratory syndrome coronavirus [J].
Yamate, M ;
Yamashita, M ;
Goto, T ;
Tsuji, S ;
Li, YG ;
Warachit, J ;
Yunoki, M ;
Ikuta, K .
MICROBES AND INFECTION, 2005, 7 (15) :1530-1540
[26]  
ZAHARIADIS G, 2003, 43 INF DIS SOC AM C
123