Enhancement of cytotoxicity against Vero E6 cells persistently infected with SARS-CoV by Mycoplasma fermentans

被引：1

作者：

Mizutani, T.

Fukushi, S.

Kenri, T.

Sasaki, Y.

Ishii, K.

Endoh, D.

Zamoto, A.

Saijo, M.

Kurane, I.

Morikawa, S.

机构：

[1] Natl Inst Infect Dis, Dept Virol 1, Tokyo 2080011, Japan

[2] Natl Inst Infect Dis, Dept Bacteriol 2, Tokyo 2080011, Japan

[3] Natl Inst Infect Dis, Dept Virol 2, Tokyo 2080011, Japan

[4] Natl Inst Infect Dis, Div Expt Anim Res, Tokyo 2080011, Japan

[5] Rakuno Gakuen Univ, Sch Vet Med, Lab Vet Radiol, Ebetsu, Hokkaido 069, Japan

来源：

ARCHIVES OF VIROLOGY | 2007年 / 152卷 / 05期

关键词：

D O I：

10.1007/s00705-006-0924-7

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We previously reported that cells with persistent severe acute respiratory syndrome coronavirus (SARS-CoV) infection were established after apoptotic events. In the present study, we investigated the cytopathic effects of dual infection with SARS-CoV and Mycoplasma fermentans on Vero E6 cells. Dual infection completely killed cells and prevented the establishment of persistent SARS-CoV infection. M. fermentans induced inhibition of cell proliferation, but the cells remained alive. Apoptosis was induced easily in M. fermentans-infected cells, indicating that they were primed for apoptosis. These results indicated that M. fermentans enhances apoptosis in surviving cells that have escaped from SARS-CoV-induced apoptosis.

引用

页码：1019 / 1025

页数：7

共 26 条

[1] MYCOPLASMA-FERMENTANS (INCOGNITUS STRAIN) INFECTION IN THE KIDNEYS OF PATIENTS WITH ACQUIRED-IMMUNODEFICIENCY-SYNDROME AND ASSOCIATED NEPHROPATHY - A LIGHT MICROSCOPIC, IMMUNOHISTOCHEMICAL, AND ULTRASTRUCTURAL-STUDY [J].

BAUER, FA ;

WEAR, DJ ;

ANGRITT, P ;

LO, SC .

HUMAN PATHOLOGY, 1991, 22 (01) :63-69

[2]

Chan PKS, 2003, EMERG INFECT DIS, V9, P1058

[3] Persistent infection of SARS coronavirus in colonic cells in vitro [J].

Chan, PKS ;

To, KF ;

Lo, AWI ;

Cheung, JLK ;

Chu, I ;

Au, FWL ;

Tong, JHM ;

Tam, JS ;

Sung, JJY ;

Ng, HK .

JOURNAL OF MEDICAL VIROLOGY, 2004, 74 (01) :1-7

[4] SPECTROPHOTOMETRIC QUANTITATION OF ANCHORAGE-DEPENDENT CELL NUMBERS USING EXTRACTION OF NAPHTHOL BLUE-BLACK-STAINED CELLULAR PROTEIN [J].

EVERITT, E ;

WOHLFART, C .

ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :122-129

[5]

Hong Tao, 2003, Zhonghua Yi Xue Za Zhi, V83, P632

[6]

ISHII K, 2007, IN PRESS VIROLOGY

[7] Use of fluorescent-protein tagging to determine the subcellular localization of Mycoplasma pneumoniae proteins encoded by the cytadherence regulatory locus [J].

Kenri, T ;

Seto, S ;

Horino, A ;

Sasaki, Y ;

Sasaki, T ;

Miyata, M .

JOURNAL OF BACTERIOLOGY, 2004, 186 (20) :6944-6955

[8] Search for the presence of six Mycoplasma species in peripheral blood mononuclear cells of subjects seropositive and seronegative for human immunodeficiency virus [J].

Kovacic, R ;

Launay, V ;

Tuppin, P ;

Lafeuillade, A ;

Feuillie, V ;

Montagnier, L ;

Grau, O .

JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (07) :1808-1810

[9] Newly discovered coronavirus as the primary cause of severe acute respiratory syndrome [J].

Kuiken, T ;

Fouchier, RAM ;

Schutten, M ;

Rimmelzwaan, GF ;

van Amerongen, G ;

van Riel, D ;

Laman, JD ;

de Jong, T ;

van Doornum, G ;

Lim, W ;

Ling, AE ;

Chan, PKS ;

Tam, JS ;

Zambon, MC ;

Gopal, R ;

Drosten, C ;

van der Werf, S ;

Escriou, N ;

Manuguerra, JC ;

Stöhr, K ;

Peiris, JSM ;

Osterhaus, ADME .

LANCET, 2003, 362 (9380) :263-270

[10] The genome sequence of the SARS-associated coronavirus [J].

Marra, MA ;

Jones, SJM ;

Astell, CR ;

Holt, RA ;

Brooks-Wilson, A ;

Butterfield, YSN ;

Khattra, J ;

Asano, JK ;

Barber, SA ;

Chan, SY ;

Cloutier, A ;

Coughlin, SM ;

Freeman, D ;

Girn, N ;

Griffith, OL ;

Leach, SR ;

Mayo, M ;

McDonald, H ;

Montgomery, SB ;

Pandoh, PK ;

Petrescu, AS ;

Robertson, AG ;

Schein, JE ;

Siddiqui, A ;

Smailus, DE ;

Stott, JE ;

Yang, GS ;

Plummer, F ;

Andonov, A ;

Artsob, H ;

Bastien, N ;

Bernard, K ;

Booth, TF ;

Bowness, D ;

Czub, M ;

Drebot, M ;

Fernando, L ;

Flick, R ;

Garbutt, M ;

Gray, M ;

Grolla, A ;

Jones, S ;

Feldmann, H ;

Meyers, A ;

Kabani, A ;

Li, Y ;

Normand, S ;

Stroher, U ;

Tipples, GA ;

Tyler, S .

SCIENCE, 2003, 300 (5624) :1399-1404

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