Detection of anti-dsDNA antibodies by computer-aided automated immunofluorescence analysis

被引：12

作者：

Lakos, Gabriella ^{[1
]}

Gonzalez, Marlene ^{[1
]}

Flaherty, David ^{[1
]}

Bentow, Chelsea ^{[1
]}

Ibarra, Claudia ^{[2
]}

Stimson, Deborah ^{[2
]}

Nacario, Lori ^{[2
]}

Hiemann, Rico ^{[3
]}

Dervieux, Thierry ^{[2
]}

机构：

[1] Inova Diagnost Inc, San Diego, CA 92182 USA

[2] Exagen Diagnost, Vista, CA USA

[3] Brandenburg Univ Technol Cottbus Senftenberg, Fac Nat Sci, Senftenberg, Germany

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2016年 / 433卷

关键词：

dsDNA; Autoantibodies; Indirect immunofluorescence; Systemic lupus erythematosus; Crithidia luciliae; DOUBLE-STRANDED DNA; CRITHIDIA-LUCILIAE; CLASSIFICATION; ASSAYS; FARR;

D O I：

10.1016/j.jim.2016.02.019

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Introduction: NOVA View is a computer aided fluorescence microscope that is used for the automated reading and interpretation of indirect immunofluorescent tests in diagnostic immunology. The objective of the present study was to evaluate the performance of the NOVA View system for the measurement of anti-dsDNA antibodies using the Crithidia luciliae indirect immunofluorescence test (CLIFT) technology. Methods: Analytical performance of NOVA View CLIFF was assessed in repeatability (within run) and reproducibility (between runs and instruments) studies. Two hundred-fifty patient samples (N = 200 consecutive samples and N = 50 samples from systemic lupus erythematosus patients) were tested to evaluate the agreement between results generated with NOVA View CLIFF, and those obtained with manual microscopic reading of the same slides. Positivity rate in SLE was assessed on the 50 SLE samples. Results: The NOVA View system showed high level of repeatability and reproducibility within runs, between runs, and between instruments. Agreement of NOVA View software interpretation and digital image reading results with manual microscopic reading results was 96.0%, and the same positivity rate was obtained on SLE samples by NOVA View digital image reading as that of manual microscopic reading (36.0% vs. 38.0%, respectively). Conclusion: Results generated by NOVA View CLIFF were equivalent to those obtained by manual microscopic reading on a large routine sample set. NOVA View demonstrated consistency within and between runs, and between instruments. Automation of CLIFF provides reliability and is a suitable alternative for routine clinical laboratories. (C) 2016 The Authors. Published by Elsevier B.V.

引用

页码：17 / 22

页数：6

共 31 条

[1] IMMUNOLOGY OF DNA .3. CRITHIDIA-LUCILIAE, A SIMPLE SUBSTRATE FOR DETERMINATION OF ANTI-DSDNA WITH IMMUNOFLUORESCENCE TECHNIQUE [J].