Colorimetric aptamer based assay for the determination of fluoroquinolones by triggering the reduction-catalyzing activity of gold nanoparticles

被引：64

作者：

Lavaee, Parirokh ^{[1
]}

Danesh, Noor Mohammad ^{[2
,3
]}

Ramezani, Mohammad ^{[2
]}

Abnous, Khalil ^{[4
,5
]}

Taghdisi, Seyed Mohammad ^{[6
,7
]}

机构：

[1] ACECR, Mashhad Branch, Mashhad, Iran

[2] Mashhad Univ Med Sci, Nanotechnol Res Ctr, Mashhad, Iran

[3] Res Inst Sci & New Technol, Mashhad, Iran

[4] Mashhad Univ Med Sci, Pharmaceut Res Ctr, Mashhad, Iran

[5] Mashhad Univ Med Sci, Sch Pharm, Dept Med Chem, Mashhad, Iran

[6] Mashhad Univ Med Sci, Targeted Drug Delivery Res Ctr, Mashhad, Iran

[7] Mashhad Univ Med Sci, Sch Pharm, Dept Pharmaceut Biotechnol, Mashhad, Iran

来源：

MICROCHIMICA ACTA | 2017年 / 184卷 / 07期

关键词：

Flower-shaped nanostructure; Ciprofloxacin; Serum; Catalytic activity; Milk; Complementary strands of aptamer; ULTRASENSITIVE DETECTION; SALMONELLA-TYPHIMURIUM; LIQUID-CHROMATOGRAPHY; ANTIBIOTIC-RESIDUES; APTASENSOR; BIOSENSOR; MILK; LUMINESCENCE; DOPAMINE; DOTS;

D O I：

10.1007/s00604-017-2213-4

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The authors describe a colorimetric assay for the detection of fluoroquinolones (FQs). It is based on the use of gold nanoparticles (AuNPs) modified with complementary DNA strands and analyte-specific FQ-binding aptamers. The modified AuNPs possess enzyme-like activity that can catalyze the reduction of nitrophenol by NaBH4. In the absence of ciprofloxacin, the flower-shape coating on the AuNPs prevents the reduction of yellow 4-nitrophenol. In the presence of ciprofloxacin, the DNA/aptamer flower leaves on the AuNPs and the AuNPs can exert their catalytic activity. This results in a color change from yellow to colorless. The assay is highly selective for FQs, fast (1 h), and has a limit of detection as low as 1.2 nM in case of ciprofloxacin. It was successfully applied to the determination of ciprofloxacin in spiked water, serum and milk samples to give LODs of 1.3, 2.6 and 3.2 nM, respectively.

引用

页码：2039 / 2045

页数：7

共 35 条

[1] Occurrence and ecological risk assessment of fluoroquinolone antibiotics in hospital waste of Lahore, Pakistan [J].