First investigation of pathogenic bacteria, protozoa and viruses in rodents and shrews in context of forest-savannah-urban areas interface in the city of Franceville (Gabon)

被引:19
作者
Mangombi, Joa Braithe [1 ,2 ,3 ]
N'dilimabaka, Nadine [1 ,4 ]
Lekana-Douki, Jean-Bernard [1 ,5 ]
Banga, Octavie [1 ]
Maghendji-Nzondo, Sydney [6 ]
Bourgarel, Mathieu [7 ,8 ]
Leroy, Eric [1 ,9 ]
Fenollar, Florence [2 ,3 ]
Mediannikov, Oleg [3 ,10 ]
机构
[1] Ctr Interdisciplinaire Rech Med Franceville CIRMF, Franceville, Gabon
[2] Aix Marseille Univ, VITROME, Microbes, AP HM,IRD, Marseille, France
[3] IHU Mediterranee Infect, Marseille, France
[4] Univ Sci & Tech Masuku USTM, Fac Sci, Dept Biol, Franceville, Gabon
[5] Univ Sci Sante USS, Dept Parasitol, Owendo, Libreville, Gabon
[6] Univ Sci Sante USS, Dept Epidemiol Biostat & Informat Med DEBIM, Owendo, Libreville, Gabon
[7] UMR ASTRE, CIRAD, Harare, Zimbabwe
[8] Univ Montpellier, INRA, CIRAD, ASTRE, Montpellier, France
[9] UMR MIVEGEC IRD CNRS UM, IRD, Montpellier, France
[10] Aix Marseille Univ, MEPHI, Microbes, AP HM,IRD, Marseille, France
关键词
ANAPLASMA-PHAGOCYTOPHILUM; COXIELLA-BURNETII; SEROLOGICAL EVIDENCE; INFECTIOUS-DISEASES; TRYPANOSOMA-LEWISI; TOXOPLASMA-GONDII; WILD RODENTS; PCR ASSAYS; URBANIZATION; PREVALENCE;
D O I
10.1371/journal.pone.0248244
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Rodents are reservoirs of numerous zoonotic diseases caused by bacteria, protozoans, or viruses. In Gabon, the circulation and maintenance of rodent-borne zoonotic infectious agents are poorly studied and are often limited to one type of pathogen. Among the three existing studies on this topic, two are focused on a zoonotic virus, and the third is focused on rodent Plasmodium. In this study, we searched for a wide range of bacteria, protozoa and viruses in different organs of rodents from the town of Franceville in Gabon. Samples from one hundred and ninety-eight (198) small mammals captured, including two invasive rodent species, five native rodent species and 19 shrews belonging to the Soricidae family, were screened. The investigated pathogens were bacteria from the Rickettsiaceae and Anaplasmataceae families, Mycoplasma spp., Bartonella spp., Borrelia spp., Orientia spp., Occidentia spp., Leptospira spp., Streptobacillus moniliformis, Coxiella bumetii, and Yersinia pestis; parasites from class Kinetoplastida spp. (Leishmania spp., Trypanosoma spp.), Piroplasmidae spp., and Toxoplasma gondii; and viruses from Paramyxoviridae, Hantaviridae, Flaviviridae and Mammarenavirus spp. We identified the following pathogenic bacteria: Anaplasma spp. (8.1%; 16/198), Bartonella spp. (6.6%; 13/198), Coxiella spp. (5.1%; 10/198) and Leptospira spp. (3.5%; 7/198); and protozoans: Piroplasma sp. (1%; 2/198), Toxoplasma gondii (0.5%; 1/198), and Trypanosoma sp. (7%; 14/198). None of the targeted viral genes were detected. These pathogens were found in Gabonese rodents, mainly Lophuromys sp., Lemniscomys striatus and Praomys sp. We also identified new genotypes: Candidatus Bartonella gabonensis and Uncultured Anaplasma spp. This study shows that rodents in Gabon harbor some human pathogenic bacteria and protozoans. It is necessary to determine whether the identified microorganisms are capable of undergoing zoonotic transmission from rodents to humans and if they may be responsible for human cases of febrile disease of unknown etiology in Gabon.
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