MCPIP1 Regulates Alveolar Macrophage Apoptosis and Pulmonary Fibroblast Activation After in vitro Exposure to Silica

被引：42
|
作者：
Wang, Xingang [1 ,2 ]
Zhang, Yuxia [1 ]
Zhang, Wei [1 ]
Liu, Haijun [1 ,3 ]
Zhou, Zewei [1 ,2 ]
Dai, Xiaoniu [1 ]
Cheng, Yusi [1 ]
Fang, Shencun [4 ]
Zhang, Yingming [4 ]
Yao, Honghong [2 ,5 ]
Chao, Jie [1 ,5 ,6 ]
机构：
[1] Southeast Univ, Sch Med, Dept Physiol, 87 Dingjiaqiao Rd, Nanjing 210009, Jiangsu, Peoples R China
[2] Southeast Univ, Sch Med, Dept Pharmacol, Nanjing 210009, Jiangsu, Peoples R China
[3] China Pharmaceut Univ, New Drug Screening Ctr, Neurobiol Lab, Nanjing 210009, Jiangsu, Peoples R China
[4] Nanjing Chest Hosp, Dept Resp Med 9, Nanjing 210029, Jiangsu, Peoples R China
[5] Southeast Univ, Key Lab Dev Genes & Human Dis, Nanjing 210096, Jiangsu, Peoples R China
[6] Southeast Univ, Sch Med, Zhongda Hosp, Dept Respirat, Nanjing 210009, Jiangsu, Peoples R China
来源：
TOXICOLOGICAL SCIENCES | 2016年 / 151卷 / 01期
基金：
中国国家自然科学基金;
关键词：
silicosis; macrophage; fibroblast; MCPIP1; apoptosis; MONOCYTE CHEMOATTRACTANT PROTEIN-1; 3-DIMENSIONAL COLLAGEN MATRICES; HUMAN FORESKIN FIBROBLASTS; ZINC-FINGER PROTEIN; ALTERNATIVE ACTIVATION; INFLAMMATORY MEDIATORS; TRANSCRIPTION FACTOR; GENE-EXPRESSION; CELL ACTIVATION; RAT LUNG;
D O I：
10.1093/toxsci/kfw029
中图分类号：
R99 [毒物学（毒理学）];
学科分类号：
100405 ;
摘要：
Results: After exposure to SiO2, MCPIP1 was significantly increased in rat AMOs. Activation and apoptosis markers in AMOs were up-regulated after exposure to SiO2. Following siRNA-mediated silencing of MCPIP1 mRNA, the markers of AMO activation and apoptosis were significantly decreased. Rat pulmonary fibroblasts (PFBs) cultured in conditional medium from AMOs treated with MCPIP1 siRNA and SiO2 showed significantly less activation and migration compared with those cultured in conditional medium from AMOs treated with control siRNA and SiO2. Conclusion: Our data suggest a vital role for MCPIP1 in AMO apoptosis and PFB activation/migration induced by SiO2.
引用
收藏
页码：126 / 138
页数：13
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