A nanostructure label-free DNA biosensor for ciprofloxacin analysis as a chemotherapeutic agent: an experimental and theoretical investigation

被引：62

作者：

Cheraghi, Somaye ^{[1
,2
]}

Taher, Mohammad A. ^{[1
]}

Karimi-Maleh, Hassan ^{[3
]}

Faghih-Mirzaei, Ehsan ^{[4
,5
]}

机构：

[1] Shahid Bahonar Univ Kerman, Dept Chem, Kerman, Iran

[2] Shahid Bahonar Univ Kerman, Young Res Soc, Kerman, Iran

[3] Quchan Univ Adv Technol, Lab Nanotechnol, Dept Chem Engn, Quchan, Iran

[4] Kerman Univ Med Sci, Fac Pharm, Dept Med Chem, Kerman, Iran

[5] Kerman Univ Med Sci, Inst Neuropharmacol, Pharmaceut Res Ctr, Kerman, Iran

来源：

NEW JOURNAL OF CHEMISTRY | 2017年 / 41卷 / 12期

关键词：

GLASSY-CARBON ELECTRODE; ELECTROCHEMICAL DETERMINATION; NANOTUBES; POLYPYRROLE; NANOPARTICLES; HYBRIDIZATION; OXIDATION; PLASMA; URINE;

D O I：

10.1039/c7nj00609h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A nanostructure DNA biosensor based on pencil graphite electrode modified with polypyrrole, single wall carbon nanotubes and ds-DNA (PGE/PP/SWCNTs/DNA) was suggested for the determination of ciprofloxacin. The proposed sensor was characterized using atomic force microscopy (AFM) and electrochemical impedance spectroscopy (EIS) methods. Guanine and adenine signals were used as the sign for investigating the interaction between ciprofloxacin and DNA at the surface of PGE/PP/SWCNTs/DNA. On the other hand, a docking simulations investigation was used for confirming the interaction between DNA and ciprofloxacin. The PGE/PP/SWCNTs/DNA demonstrates a wide dynamic range (0.008-30.0 mu M) with an ultrasensitive detection limit of 4.0 nM for the determination of ciprofloxacin using the differential pulse voltammetric (DPV) method. PGE/PP/SWCNTs/DNA showed a good ability for the analysis of ciprofloxacin in drug and urine samples.

引用

页码：4985 / 4989

页数：5

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