Preparation, chemical structure, and immunostimulatory activity of a water-soluble heteropolysaccharide from Suillus granulatus fruiting bodies

被引：7

作者：

Gao, Xiong ^{[1
]}

Zeng, Ranhua ^{[3
]}

Ho, Chi-Tang ^{[4
]}

Li, Bin ^{[3
,5
]}

Chen, Shaodan ^{[1
]}

Xiao, Chun ^{[1
]}

Hu, Huiping ^{[1
]}

Cai, Manjun ^{[1
]}

Chen, Zhongzheng ^{[3
,5
]}

Xie, Yizhen ^{[1
,2
]}

Wu, Qingping ^{[1
]}

机构：

[1] Guangdong Acad Sci, Inst Microbiol, State Key Lab Appl Microbiol Southern China, Guangdong Prov Key Lab Microbial Safety & Hlth, Guangzhou 510070, Peoples R China

[2] Guangdong Yuewei Biotechnol Co Ltd, Zhaoqing 526000, Peoples R China

[3] South China Agr Univ, Coll Food Sci, 483 Wushan St, Guangzhou 510642, Peoples R China

[4] Rutgers State Univ, Dept Food Sci, Dudley Rd, New Brunswick, NJ 08901 USA

[5] South China Agr Univ, Guangdong Prov Key Lab Nutraceuticals & Funct Foo, Guangzhou 510642, Peoples R China

来源：

FOOD CHEMISTRY-X | 2022年 / 13卷

关键词：

Suillus granulatus; Heteropolysaccharide; Structural characterization; Immunomodulatory activity; NF-KAPPA-B; SIGNALING PATHWAYS; MACROPHAGE ACTIVATION; ANTITUMOR-ACTIVITY; POLYSACCHARIDE; ANTIOXIDANT; GLUCAN; MECHANISM; CELLS;

D O I：

10.1016/j.fochx.2022.100211

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A water-soluble heteropolysaccharide (SGP2-1) was purified from Suillus granulatus fruiting bodies by anionexchange chromatography and gel permeation chromatography. The structural characteristics were analyzed by high-performance gel permeation chromatography, high-performance liquid chromatography, Fourier transform infrared spectroscopy, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectroscopy. The immunostimulatory activity was investigated using RAW 264.7 macrophages. Results showed that SGP2-1 with weight average molecular weight of 150.75 kDa was composed of mannose, glucose, and xylose. The backbone of SGP2-1 was mainly composed of -> 4)-alpha-Glcp-(1 ->, and the terminal group alpha-D-Glcp -> was linked to the main chain by 0-6 position. SGP2-1 could significantly enhance pinocytic capacity, reactive oxygen species production, and cytokines secretion. SGP2-1 exerted immunomodulatory effects through interacting with toll-like receptor 2, and activating mitogen-activated protein kinase, phosphatidylinositol-3-kinase/protein kinase B, and nuclear factor-kappa B signaling pathways. These findings indicated that SGP2-1 could be explored as a potential immunomodulatory agent for application in functional foods.

引用

页数：10

共 41 条

[1] An α-glucan of Pseudallescheria boydii is involved in fungal phagocytosis and toll-like receptor activation [J].