Effect of spleen tyrosine kinase on nonsmall cell lung cancer

被引：2

作者：

Hao, Ying Tao ^{[1
]}

Peng, Chuan Liang ^{[1
]}

Zhao, Yun Peng ^{[1
]}

Sun, Qi Feng ^{[1
]}

Zhao, Xiao Gang ^{[1
]}

Cong, Bo ^{[1
]}

机构：

[1] Shandong Univ, Hosp 2, Dept Thorac, 247 Beiyuan St, Jinan 250033, Shandong, Peoples R China

来源：

JOURNAL OF CANCER RESEARCH AND THERAPEUTICS | 2018年 / 14卷

关键词：

Invasion; matrix metalloproteinase; nonsmall cell lung cancer; spleen tyrosine kinase; PATIENTS SURVIVAL; SYK; EXPRESSION; METALLOPROTEINASE-2; METHYLATION; ACTIVATION; INHIBITORS;

D O I：

10.4103/0973-1482.174170

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Aim of Study: To investigate the anti-tumor effect of spleen tyrosine kinase (Syk) on the human nonsmall cell lung cancer cells in vitro and its mechanism. Materials and Methods: In this study, we constructed a eukaryotic expression vector pcDNA3.1D/V5-His-TOPO/ Syk and transfected it into human nonsmall cell lung cancer cells A549. Then, we not only analyzed the expression of Syk in transfected cells and its invasion but also the expression of matrix metalloproteinase-9 (MMP-9). Results: The results showed that overexpressed Syk significantly inhibited A549 cell invasive ability by decreasing the expression of MMP-9. Conclusion: The overexpressed Syk plays an important role in tumor invasion and metastasis, and a negative role in human nonsmall cell lung cancer cells.

引用

页码：S100 / S104

页数：5

共 16 条

[1] The Syk family of protein tyrosine kinases in T-cell activation and development [J].

Chu, DH ;

Morita, CT ;

Weiss, A .

IMMUNOLOGICAL REVIEWS, 1998, 165 :167-180

[2] Research on the reactivation of Syk expression caused by the inhibition of DNA promoter methylation in the lung cancer [J].

Dong, Shangwen ;

Ma, L. ;

Xu, N. ;

Yan, H. Q. ;

Liu, H. Y. ;

Li, Y. W. ;

Zhang, R. .

NEOPLASMA, 2011, 58 (01) :89-95

[3] Selective, novel spleen tyrosine kinase (Syk) inhibitors suppress chronic lymphocytic leukemia B-cell activation and migration [J].

Hoellenriegel, J. ;

Coffey, G. P. ;

Sinha, U. ;

Pandey, A. ;

Sivina, M. ;

Ferrajoli, A. ;

Ravandi, F. ;

Wierda, W. G. ;

O'Brien, S. ;

Keating, M. J. ;

Burger, J. A. .

LEUKEMIA, 2012, 26 (07) :1576-1583

[4] The non-receptor-associated tyrosine kinase Syk is a regulator of metastatic behavior in human melanoma cells [J].

Hoeller, C ;

Thallinger, C ;

Pratscher, B ;

Bister, MD ;

Schicher, N ;

Loewe, R ;

Heere-Ress, E ;

Roka, F ;

Sexl, V ;

Pehamberger, H .

JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2005, 124 (06) :1293-1299

[5]

Koshikawa N, 2001, KEIO J MED, V50, P45

[6] Molecular mechanisms in B cell antigen receptor signaling [J].

Kurosaki, T .

CURRENT OPINION IN IMMUNOLOGY, 1997, 9 (03) :309-318

[7] Syk Tyrosine Kinase Acts as a Pancreatic Adenocarcinoma Tumor Suppressor by Regulating Cellular Growth and Invasion [J].

Layton, Tracy ;

Stalens, Cristel ;

Gunderson, Felizza ;

Goodison, Steve ;

Silletti, Steve .

AMERICAN JOURNAL OF PATHOLOGY, 2009, 175 (06) :2625-2636

[8]

Ma L, 2010, CLIN LAB, V56, P407

[9] Protein expression of matrix metalloproteinases 2 and 9 and tissue inhibitors of metalloproteinase 1 and 2 in papillary thyroid carcinomas [J].

Maeta, H ;

Ohgi, S ;

Terada, T .

VIRCHOWS ARCHIV-AN INTERNATIONAL JOURNAL OF PATHOLOGY, 2001, 438 (02) :121-128

[10] Clinical significance of nuclear expression of spleen tyrosine kinase (Syk) in gastric cancer [J].

Nakashima, Hiroshi ;

Natsugoe, Shoji ;

Ishigami, Sumiya ;

Okumura, Hiroshi ;

Matsumoto, Masataka ;

Hokita, Shuichi ;

Aikou, Takashi .

CANCER LETTERS, 2006, 236 (01) :89-94

← 1 2 →