Determination of amlodipine using terbium-sensitized luminescence in the presence of europium(III) as a co-luminescence reagent

A sensitive time-resolved luminescence method for the determination of amlodipine (AM) in methanol and in aqueous solution is described. The method is based on the luminescence sensitization of terbium (Tb3+) by formation of a ternary complex with AM in the presence of tri-n-octylphosphine oxide (TOPO) as co-ligand, dodecylbenzenesulfate as surfactant and europium ion as a co-luminescence reagent. The signal for Tb-AM-TOPO is monitored at (ex)=242 nm and (em)=550 nm. Optimum conditions for the formation of the complex in aqueous system were 0.015 m Tris (hydroxylmethyl) amino methane buffer, pH 9.0, TOPO (1.0x10(-4) m), Eu3+ (2.0x10(-7) m), dodecylbenzenesulfate (0.14%) and 6.0x10(-5) m of Tb3+, which allows the determination of 10-50 ppb of AM with a limit of detection of 1.2 ppb. The relative standard deviations of the method range between 0.1 and 0.2% indicated excellent reproducibility of the method. The proposed method was successfully applied for the assay of AM in pharmaceutical formulations and in plasma samples. Average recoveries of 98.5 +/- 0.2% and 95.2 +/- 0.2% were obtained for AM in tablet and plasma samples respectively. Copyright (c) 2013 John Wiley & Sons, Ltd.