Dapdiamides, Tripeptide Antibiotics Formed by Unconventional Amide Ligases

被引：36

作者：

Dawlaty, Jessica ^{[1
]}

Zhang, Xiaorong ^{[1
]}

Fischbach, Michael A. ^{[2
,3
]}

Clardy, Jon ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA

[2] Univ Calif San Francisco, Dept Bioengn & Therapeut Sci, San Francisco, CA 94158 USA

[3] Univ Calif San Francisco, Calif Inst Quantitat Biol, San Francisco, CA 94158 USA

来源：

JOURNAL OF NATURAL PRODUCTS | 2010年 / 73卷 / 03期

关键词：

BIOSYNTHETIC GENE-CLUSTER; ESCHERICHIA-COLI; ASPARAGINE SYNTHETASE; IRON ENZYME; ACID; INACTIVATION; CONDENSATION;

D O I：

10.1021/np900685z

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Construction of a genomic DNA library from Pantoea agglomerans strain CU0119 and screening against the plant pathogen Erwinia amylovora yielded a new family of antibiotics, dapdiamides A-E (1-5). The structures were established through 2D-NMR experiments and mass spectrometry, as well as the synthesis of dapdiamide A (1). Transposon mutagenesis of the active cosmid allowed identification of the biosynthetic gene cluster. The dapdiamide family's promiscuous biosynthetic pathway contains two unconventional amide ligases that are predicted to couple its constituent monomers.

引用

页码：441 / 446

页数：6

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