Influence of phenolic profile on the RP-HPLC detection and anti-carcinogenic potential of cherry laurel extracts from Black Sea Region-Turkey

被引：4
作者：
Bayrambas, Kadir [1 ]
Cakir, Bilal [2 ,3 ]
Gulseren, Ibrahim [4 ]
机构：
[1] Untas Food Co AS, Merkez, Cankiri, Turkey
[2] Istanbul Sabahattin Zaim Univ IZU, Halal Food R&D Ctr, Istanbul, Turkey
[3] Marmara Univ, Fac Pharm, Inst Hlth Sci, Dept Biochem, Istanbul, Turkey
[4] Istanbul Sabahattin Zaim Univ IZU, Dept Food Engn, Halkalt Campus, TR-34303 Istanbul, Turkey
来源：
MICROCHEMICAL JOURNAL | 2019年 / 149卷
关键词：
Cherry laurel; Phenolic content; Chlorogenic acid; RP-HPLC; HCT-116; cells; Cell culture; LAUROCERASUS-OFFICINALIS ROEM; ANTIOXIDANT ACTIVITY; FRUIT; ACID; POLYPHENOLS; APOPTOSIS; CAPACITY; CURCUMIN; CELLS; TEA;
D O I：
10.1016/j.microc.2019.103963
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Cherry laurel produces edible dark colored fruits that are anticipated to have strongly antimddative properties. Various studies dealt with the phenolic composition of cherry laurel fruits or concentrated juice to analyze the antioxidative potential. Here, in addition to polyphenol analysis, anti-proliferative activities of cherry laurel components have been investigated. Using an RP-HPLC method, phenolic content of the fruits were analyzed and 14 different polyphenolic compounds were determined in three varieties (55K06, 61K04 and 61K05), some of which were detected for the first time in cherry laurel fruits. Chlorogenic acid was found to be the major phenolic compound. In cell culture studies, polyphenolic extracts demonstrated anti-carcinogenic activity against HCT-116 colon carcinoma cells, potentially due to apoptotic activity of the extracts as demonstrated by flow cytometry. The composition and concentration of phenolic compounds largely determined the extent of cellular viability and apoptotic potential.
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页数：8
相关论文
共 52 条
[1]   Compositional characteristics and antioxidant components of cherry laurel varieties and pekmez [J].
Alasalvar, C ;
Al-Farsi, M ;
Shahidi, F .
JOURNAL OF FOOD SCIENCE, 2005, 70 (01) :S47-S52
[2]   Flavonoid and chlorogenic acid levels in apple fruit: characterisation of variation [J].
Awad, MA ;
de Jager, A ;
van Westing, LM .
SCIENTIA HORTICULTURAE, 2000, 83 (3-4) :249-263
[3]   Phenolic compounds in plants and agri-industrial by-products: Antioxidant activity, occurrence, and potential uses [J].
Balasundram, Nagendran ;
Sundram, Kalyana ;
Samman, Samir .
FOOD CHEMISTRY, 2006, 99 (01) :191-203
[4]  
Baytop T., 1999, CURING TURKISH PLANT
[5]  
BRATTAIN MG, 1981, CANCER RES, V41, P1751
[6]   Dissolution kinetics of polyphenol bearing calcium pectate hydrogels in simulated gastric or intestinal media and their anti-carcinogenic capacities [J].
Cakir, Bilal ;
Gulseren, Ibrahim .
FOOD HYDROCOLLOIDS, 2017, 70 :69-75
[7]   Some physico-mechanic properties of cherry laurel (Prunus lauracerasus L.) fruits [J].
Çalisir, S ;
Aydin, C .
JOURNAL OF FOOD ENGINEERING, 2004, 65 (01) :145-150
[8]   Antiproliferative activity of New Zealand propolis and phenolic compounds vs human colorectal adenocarcinoma cells [J].
Catchpole, Owen ;
Mitchell, Kevin ;
Bloor, Stephen ;
Davis, Paul ;
Suddes, Amanda .
FITOTERAPIA, 2015, 106 :167-174
[9]   Estimation of Certain Physical and Chemical Fruit Characteristics of Various Cherry Laurel (Laurocerasus officinalis Roem.) Genotypes [J].
Celik, Ferit ;
Ercisli, Sezai ;
Yilmaz, Suzan O. ;
Hegedus, Attila .
HORTSCIENCE, 2011, 46 (06) :924-927
[10]   FEATURES OF APOPTOTIC CELLS MEASURED BY FLOW-CYTOMETRY [J].
DARZYNKIEWICZ, Z ;
BRUNO, S ;
DELBINO, G ;
GORCZYCA, W ;
HOTZ, MA ;
LASSOTA, P ;
TRAGANOS, F .
CYTOMETRY, 1992, 13 (08) :795-808
123456