Antimicrobial PCMX facilitates the volatile fatty acids production during sludge anaerobic fermentation: Insights of the interactive principles, microbial metabolic profiles and adaptation mechanisms

被引:63
|
作者
Du, Wei [1 ,2 ]
Wang, Feng [1 ,2 ]
Fang, Shiyu [1 ,2 ]
Huang, Wenxuan [1 ,2 ]
Cheng, Xiaoshi [1 ,2 ]
Cao, Jiashun [1 ,2 ]
Fang, Fang [1 ,2 ]
Wu, Yang [3 ]
Luo, Jingyang [1 ,2 ,4 ]
机构
[1] Hohai Univ, Key Lab Integrated Regulat & Resource Dev Shallow, Minist Educ, 1 Xikang Rd, Nanjing 210098, Peoples R China
[2] Hohai Univ, Coll Environm, 1 Xikang Rd, Nanjing 210098, Peoples R China
[3] Tongji Univ, Sch Environm Sci & Engn, State Key Lab Pollut Control & Resource Reuse, 1239 Siping Rd, Shanghai 200092, Peoples R China
[4] Anhui Prov Key Lab Environm Pollut Control & Resou, Hefei, Peoples R China
基金
中国国家自然科学基金; 中国博士后科学基金;
关键词
Para-chloro; Para-chloro-meta -xylenol (PCMX); Volatile fatty acids (VFAs); Dose-dependent effects; Interaction; Metabolic functions; Microbial adaptation; ACCUMULATION; ENHANCEMENT; SYSTEM; IMPACT;
D O I
10.1016/j.cej.2022.137339
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Para-chloro-meta-xylenol (PCMX), as a massively used antimicrobial agent, exhibited dose-dependent effects on the sludge fermentation for volatile fatty acids (VFAs). The presence of PMCX at environmental levels (10-20 mg/g TSS) promoted the VFAs production by 2.5-3.0 folds but caused evident inhibition at high level (100 mg/g TSS). PCMX synchronously promoted the sludge solubilization, hydrolysis, and acidification but inhibited methanogenesis. Mechanistic investigations revealed that PCMX could interact with the extracellular polymeric substances (EPS) in sludge and cause the release of biodegradable substrates (especially proteins) by altering the WAS structures. Moreover, the functional fermentative bacteria (i.e., Firmicutes and Chloroflexi) were enriched while those VFAs consumers (i.e., Methanothrix and Methanomassiliicoccus) were reduced with low level of PCMX exposure. The microbial enzymatic activities (i.e., protease) and critical genes relevant to the substrates transport (i.e., gluC and lhpN) and metabolisms (especially the proteins, i.e., pckA), and VFAs biosynthesis (i.e., fabG) were all upregulated with the low PCMX stimulus. The microbial response and adaptation to PCMX toxicity were mainly attributed to the regulation of two-component and quorum sensing systems. Overall, PCMX contributed to the VFAs accumulation during sludge fermentation by mainly interacting and disrupting the sludge structures, and improving the microbial metabolic function traits. This work provided new insights into exogenous pol-lutants' interactions, potential influences, and reaction mechanisms in the complicated sludge matrix.
引用
收藏
页数:11
相关论文
共 50 条
  • [41] Influence of perfluorooctanoic acid on alkaline anaerobic fermentation of waste activated sludge: Perspective from volatile fatty acids production and sludge reduction
    Zhao, Zhilin
    Zheng, Xiaoying
    Yang, Shanshan
    He, Haidong
    Han, Zongshuo
    Li, Wenfei
    Lin, Tao
    Xu, Hang
    JOURNAL OF ENVIRONMENTAL MANAGEMENT, 2024, 370
  • [42] Ultrasonic-assisted hypochlorite activation accelerated volatile fatty acids production during sewage sludge fermentation: Critical insights on solubilization/hydrolysis stages and microbial traits
    Zhu, Rui
    Zhao, Shanshan
    Ju, Chenlu
    Yang, Qing
    Cui, Chengcheng
    Wu, Lijuan
    Wang, Min
    Feng, Leiyu
    Wu, Yang
    BIORESOURCE TECHNOLOGY, 2023, 383
  • [43] Upgrading volatile fatty acids production from anaerobic co-fermentation of orange peel waste and sewage sludge: Critical roles of limonene on functional consortia and microbial metabolic traits
    Shao, Qianqi
    Zhang, Qin
    Fang, Shiyu
    Huang, Wenxuan
    Li, Ziyu
    Fang, Xinyang
    Bao, Xingchen
    Lin, Lifang
    Cao, Jiashun
    Luo, Jingyang
    BIORESOURCE TECHNOLOGY, 2022, 362
  • [44] Metagenomic and proteomic insights into graphene oxide-boosted anaerobic co-fermentation of food waste and sewage sludge for volatile fatty acids production
    Zhao, Yinlan
    Yuan, Feiyi
    Su, Yu
    Wu, Meirou
    Li, Xiaolu
    Feng, Dianlei
    Dong, Haiqing
    Mustafa, Ahmed M.
    Luo, Jingyang
    Feng, Leiyu
    Chen, Yinguang
    CHEMICAL ENGINEERING JOURNAL, 2025, 504
  • [45] Tannic Acid Modulation of Substrate Utilization, Microbial Community, and Metabolic Traits in Sludge Anaerobic Fermentation for Volatile Fatty Acid Promotion
    Huang, Wenxuan
    Wang, Feng
    Xia, Xue
    Fang, Shiyu
    Cheng, Xiaoshi
    Zhou, Aijuan
    Feng, Leiyu
    Wang, Dongbo
    Luo, Jingyang
    ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2024, 58 (22) : 9792 - 9803
  • [46] Effects of sulfite and freezing co-pretreatment on the volatile fatty acids production of waste activated sludge in anaerobic fermentation
    Yang, Li-Li
    Zhang, Yong
    Wang, Hui
    Huang, Jian
    Xiao, Yi-Fan
    Liu, Jia-Qi
    Li, Can
    Hu, Ding-Cheng
    Yan, Chuan-Chuan
    JOURNAL OF WATER PROCESS ENGINEERING, 2024, 65
  • [47] Metagenomic insights into improving mechanisms of Fe0 nanoparticles on volatile fatty acids production from potato peel waste anaerobic fermentation
    Yang, Guang
    Xu, Chonglin
    Varjani, Sunita
    Zhou, Yaoyu
    Wong, Jonathan W. C.
    Duan, Guilan
    BIORESOURCE TECHNOLOGY, 2022, 361
  • [48] Insights into thermal hydrolysis pretreatment temperature for enhancing volatile fatty acids production from sludge fermentation: Performance and mechanism
    Xiang, Zhuangzhuang
    Huang, Xiao
    Chen, Hanfeng
    Liu, Biming
    Liu, Ziming
    Dong, Wenyi
    Wang, Hongjie
    BIORESOURCE TECHNOLOGY, 2023, 379
  • [49] Insights into the effect of nitrate photolysis on short-chain fatty acids production from waste activated sludge in anaerobic fermentation system: Performance and mechanisms
    Liu, Zhihong
    Cui, Zhixuan
    Guo, Zhengtong
    Li, Dengfei
    He, Zhangwei
    Liu, Wenzong
    Yue, Xiuping
    Zhou, Aijuan
    WATER RESEARCH, 2024, 258
  • [50] Combining radio frequency heating and alkaline treatment for enhancement of sludge disintegration and volatile fatty acids production from anaerobic fermentation
    Chen, Kai
    Zhang, Jing
    Li, Zhuo
    Wang, Dongquan
    Zeng, Bizhen
    Chen, Wangyang
    Yang, Lisha
    Zhai, Shixin
    Zhu, Hongtao
    BIORESOURCE TECHNOLOGY, 2024, 412