Continuous Detection Method for Ractopamine with Surface Plasmon Resonance Biosensor

被引：1

作者：

Li Hui ^{[1
]}

Cai Hao-Yuan ^{[1
]}

Chen Xing ^{[1
]}

Sun Jian-Hai ^{[1
]}

Zhang Lu-Lu ^{[1
]}

Cui Da-Fu ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Elect, State Key Lab Transducer Tech, Beijing 100190, Peoples R China

来源：

CHINESE JOURNAL OF ANALYTICAL CHEMISTRY | 2010年 / 38卷 / 03期

关键词：

Surface plasmon resonance; Biosensor; Kinetics; Continuous detection; Ractopamine; SULFAMETHAZINE; RESIDUES;

D O I：

10.3724/SP.J.1096.2010.00381

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The interaction between the anti-Ractopamine (Rac) monoclonal antibody and the Rac derivation immobilized on the sensor chip surface was studied with surface plasmon resonance (SPR) biosensor. A continuous detection method based on the linear response during the association phase was developed. The sensor chip surface was regenerated after several tests performed continuously, thus the detection step was simplified and the life span of the chip was prospected to be prolonged. The detection was performed as an inhibitive immunoassay. The mixture of anti-Rac monoclonal antibody and the sample flowed over the surface with Rac derivation was immobilized. The relative response was in inversely proportion to the concentration of Rac. The detection limit was less than 4 mu g/L with a detection time of 15 min.

引用

页码：381 / 384

页数：4

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