Synthesis of Circular Double-Stranded DNA Having Single-Stranded Recognition Sequence as Molecular-Physical Probe for Nucleic Acid Hybridization Detection Based on Atomic Force Microscopy Imaging

被引：1

作者：

Nakano, Koji ^{[1
]}

Matsunaga, Hideshi ^{[1
]}

Murata, Masaharu ^{[2
]}

Soh, Nobuaki ^{[1
]}

Imato, Toshihiko ^{[1
]}

机构：

[1] Kyushu Univ, Fac Engn, Dept Appl Chem, Nishi Ku, Fukuoka 8190395, Japan

[2] Kyushu Univ, Fac Med Sci, Dept Adv Med Initiat, Higashi Ku, Fukuoka 8128582, Japan

来源：

ANALYTICAL SCIENCES | 2009年 / 25卷 / 08期

基金：

日本科学技术振兴机构;

关键词：

GOLD; MONOLAYERS; NANOPARTICLES; SPECIFICITY; SURFACES; BINDING;

D O I：

10.2116/analsci.25.993

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new class of DNA probes having a mechanically detectable tag is reported. The DNA probe, which consists of a single-stranded recognition sequence and a double-stranded circular DNA entity, was prepared by polymerase reaction. M13mp18 single strand and a 32mer oligodeoxynucleotide whose 5'-end is decorated with the recognition sequence were used in combination as template and primer, respectively. We have successfully demonstrated that the DNA probe is useful for bioanalytical purposes: by deliberately attaching target DNA molecules onto Au(111) substrates and by mechanically reading out the tag-entity using a high-resolution microscopy including atomic force microscopy, visualization/detection of the individual target/probe DNA conjugate was possible simply yet straightforwardly. The present DNA probe can be characterized as a 100%-nucleic acid product material. It is simply available by one-pod synthesis. A surface topology parameter, image roughness, has witnessed its importance as a quantitative analysis index with particular usability in the present visualization/detection method.

引用

页码：993 / 998

页数：6