Differential changes of glutathione levels in astrocytes and neurons in ischemic brains by two-photon imaging

被引：23

作者：

Bragin, Denis E. ^{[2
,3
]}

Zhou, Bo ^{[4
,5
]}

Ramamoorthy, Prabhu

Mueller, Wolfgang S. ^{[2
,3
]}

Connor, John A. ^{[3
,6
]}

Shi, Honglian ^{[1
]}

机构：

[1] Univ Kansas, Dept Pharmacol & Toxicol, Sch Pharm, Lawrence, KS 66045 USA

[2] Univ New Mexico, Dept Neurosurg, Albuquerque, NM 87131 USA

[3] UNM, BRaIN Imaging Ctr, Albuquerque, NM USA

[4] Zhejiang Univ, Dept Neurosurg, Affiliated Hosp 2, Hangzhou 310003, Zhejiang, Peoples R China

[5] Zhejiang Univ, Inst Brain Med, Hangzhou 310003, Zhejiang, Peoples R China

[6] Univ New Mexico, Dept Neurosci, Albuquerque, NM 87131 USA

来源：

JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM | 2010年 / 30卷 / 04期

关键词：

astrocyte; GSH; MCAO; neuron; penumbra; stroke; CEREBRAL-ARTERY OCCLUSION; CEREBROSPINAL-FLUID; REDOX STATUS; HYPOXIA; CELLS;

D O I：

10.1038/jcbfm.2010.9

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Using two-photon imaging techniques with monochlorobimane as a glutathione (GSH) probe, we investigated GSH levels in both core and penumbra regions of an ischemic brain after middle cerebral artery occlusion. We found that the GSH level significantly decreased in the ischemic core, but increased significantly in the penumbra. Furthermore, we observed a differential change of the GSH levels in neurons and astrocytes in the penumbra. The GSH level in neurons increased significantly whereas it decreased slightly in astrocytes in the penumbra. These findings reveal critical region and cell type-dependent changes of the GSH level in an ischemic brain. Journal of Cerebral Blood Flow & Metabolism (2010) 30, 734-738; doi:10.1038/jcbfm.2010.9; published online 27 January 2010

引用

页码：734 / 738

页数：5

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