Susceptibility of ATM-deficient pancreatic cancer cells to radiation

被引：28

作者：

Ayars, Michael ^{[1
]}

Eshleman, James ^{[1
,2
]}

Goggins, Michael ^{[1
,2
,3
]}

机构：

[1] Johns Hopkins Univ, Sch Med, Dept Pathol, Baltimore, MD 21205 USA

[2] Johns Hopkins Univ, Sch Med, Dept Oncol, Baltimore, MD 21205 USA

[3] Johns Hopkins Univ, Sch Med, Dept Med, Sol Goldman Pancreat Canc Res Ctr, Baltimore, MD 21205 USA

来源：

CELL CYCLE | 2017年 / 16卷 / 10期

关键词：

ATM; pancreatic cancer; radiosensitivity; STEREOTACTIC BODY RADIOTHERAPY; CHRONIC LYMPHOCYTIC-LEUKEMIA; PREDISPOSITION GENES; PROTEIN EXPRESSION; CELLULAR-RESPONSE; DNA-REPAIR; PHASE-II; IN-VITRO; MUTATIONS; SURVIVAL;

D O I：

10.1080/15384101.2017.1312236

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Ataxia telangiectasia mutated (ATM) is inactivated in a significant minority of pancreatic ductal adenocarcinomas and may be predictor of treatment response. We determined if ATM deficiency renders pancreatic cancer cells more sensitive to fractionated radiation or commonly used chemotherapeutics. ATM expression was knocked down in three pancreatic cancer cell lines using ATM-targeting shRNA. Isogenic cell lines were tested for sensitivity to several chemotherapeutic agents and radiation. DNA repair kinetics were analyzed in irradiated cells using the comet assay. We find that while rendering pancreatic cancer cells ATM-deficient did not significantly change their sensitivity to several chemotherapeutics, it did render them exquisitely sensitized to radiation. Pancreatic cancer ATM status may help predict response to radiotherapy.

引用

页码：991 / 998

页数：8

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