Synthesis and functionalities of poly(N-vinylalkylamide) .7. A novel aqueous two-phase systems based on poly(N-vinylacetamide) and dextran

被引：0

作者：

Kishida, A ^{[1
]}

Nakano, S ^{[1
]}

Kikunaga, Y ^{[1
]}

Akashi, M ^{[1
]}

机构：

[1] KAGOSHIMA UNIV,FAC ENGN,DEPT APPL CHEM & CHEM ENGN,KAGOSHIMA 890,JAPAN

来源：

JOURNAL OF APPLIED POLYMER SCIENCE | 1998年 / 67卷 / 02期

关键词：

poly(N-vinylacetamide); aqueous two-phase system; dextran; poly(ethylene glycol); myoglobin;

D O I：

10.1002/(SICI)1097-4628(19980110)67:2<255::AID-APP7>3.0.CO;2-T

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

It was found that a poly(N-vinylacetamide) (polyNVA)-dextran system formed two phases. The top was polyNVA-rich and the bottom phase was dextran-rich, which is the same as the poly(ethylene glycol) (PEG)-dextran system, and the phase separation occurred at a lower concentration than the PEG-dextran system. The protein separation in the polyNVA-dextran system was studied using myoglobin. Myoglobin was partitioned more in the bottom (dextran-rich) phase in a polyNVA-dextran system. The partition coefficient of the polyNVA-dextran system was smaller that of the PEG-dextran system, which suggests that the polyNVA-dextran system is superior to the PEG-dextran system for myoglobin separation. (C) 1998 John Wiley & Sons, Inc.

引用

页码：255 / 258

页数：4

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