Covalent immobilization of lipases on monodisperse magnetic microspheres modified with PAMAM-dendrimer

被引：35

作者：

Zhu, Weiwei ^{[1
]}

Zhang, Yimei ^{[2
,3
]}

Hou, Chen ^{[1
]}

Pan, Duo ^{[1
]}

He, Jianjun ^{[1
]}

Zhu, Hao ^{[1
,2
,3
]}

机构：

[1] Lanzhou Univ, State Key Lab Appl Organ Chem, Key Lab Nonferrous Met Chem & Resources Utilizat, Coll Chem & Chem Engn,Inst Biochem Engn & Environ, Lanzhou 730000, Peoples R China

[2] North China Elect Power Univ, Suzhou Res Acad, Suzhou 215000, Peoples R China

[3] North China Elect Power Univ, Environm Res Acad, Beijing 100000, Peoples R China

来源：

JOURNAL OF NANOPARTICLE RESEARCH | 2016年 / 18卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Magnetic nanoparticles; Lipase immobilization; Dendritic modification; ENZYME IMMOBILIZATION; GLUTARALDEHYDE ACTIVATION; NANOPARTICLES; SUPPORTS; POLYMERS; STRATEGY; SIZE;

D O I：

10.1007/s11051-016-3337-x

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

This paper reported an immobilization of Candida rugosa lipase (CRL) onto PAMAM-dendrimer-grafted magnetic nanoparticles synthesized by a modified solvothermal reduction method. The dendritic magnetic nanoparticles were amply characterized by several instrumental measurements, and the CRL was covalently anchored on the three generation supports with glutaraldehyde as coupling reagent. The amount of immobilized enzyme was up to 150 mg/g support and the factors related with the enzyme activity were investigated. The immobilization of lipase improved their performance in wider ranges of pH and temperature. The immobilized lipase exhibited excellent thermal stability and reusability in comparison with free enzyme and can be reused 10 cycles with the enzymatic activity remained above 90 %. The properties of lipase improved obviously after being immobilized on the dendritic supports. The inactive immobilized lipase could be regenerated with glutaraldehyde and Cu2+, respectively. This synthetic strategy was facile and eco-friendly for applications in lipase immobilization.

引用

页码：1 / 13

页数：13

共 43 条

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