Quorum sensing modulation and inhibition in biofilm forming foot ulcer pathogens by selected medicinal plants

被引:7
作者
Mashamba, Tebatso G. [1 ]
Adeosun, Idowu J. [1 ]
Baloyi, Itumeleng T. [1 ]
Tshikalange, Emmanuel T. [2 ]
Cosa, Sekelwa [1 ]
机构
[1] Univ Pretoria, Dept Biochem Genet & Microbiol, Div Microbiol, Private Bag X20,Hat field, ZA-0028 Pretoria, South Africa
[2] Univ Pretoria, Dept Plant & Soil Sci, Lynnwood Rd,Private Bag X20,Hat field, ZA-0028 Pretoria, South Africa
基金
新加坡国家研究基金会; 英国医学研究理事会;
关键词
Aloe ferox; Antiquorum sensing; Biofilm inhibition; Euclea natalensis; Multi-drug resistant pathogens; Molecular modelling; ANTIBIOFILM ACTIVITY; ESSENTIAL OILS; EXTRACTS; ANTIBACTERIAL; MECHANISM; DOCKING;
D O I
10.1016/j.heliyon.2022.e09303
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The crisis of antibiotic resistance necessitates the search of phytochemicals as potential antibacterial, anti-quorum sensing and antibiofilm forming agents. For the present study, fifteen (15) selected medicinal plants were evaluated to inhibit the biological activities of multi-drug resistant (MDR) pathogenic bacteria (Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis) associated with diabetic foot ulcer. Antibacterial activities revealed noteworthy minimum inhibitory concentration (MIC) values <1 mg/mL for thirteen (13) out of the sixty (60) plant extracts screened. The potent extracts included Euclea natalensis ethyl acetate (0.25 mg/mL), Aloe ferox methanol (0.5 mg/ml) and Warburgia salutaris aqueous (0.5 mg/mL) extracts. Chemical profiling of the active extracts using gas chromatography-mass spectrometry (GC-MS) identified neophytadiene, guanosine, squalene, cis megastigma-5,8-diene-4-one and sorbitol as prevalent compounds among the active extracts. Antiquorum sensing activities of E. natalensis (ethyl acetate), A. ferox (methanol) and W. salutaris (aqueous) extracts ranged from 4.81 - 58.34% with E. natalensis (ethyl-acetate) showing the highest activity. Molecular docking against CviR protein showed selected compounds having high docking scores with sorbitol showing the highest score of -7.04 kcal/mol. Warburgia salutaris aqueous extract exhibited the highest biofilm inhibition (73%) against E. coli. Euclea natalensis, Aloe ferox and Warburgia salutaris compounds act as antagonist of N-acyl homoserine lactone (AHL) signaling, thus may serve as candidates in antipathogenic and antibiofilm phytomedicine development for MDR foot ulcer bacterial pathogens.
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页数:11
相关论文
共 51 条
[11]   A Strategy for Antagonizing Quorum Sensing [J].
Chen, Guozhou ;
Swem, Lee R. ;
Swem, Danielle L. ;
Stauff, Devin L. ;
O'Loughlin, Colleen T. ;
Jeffrey, Philip D. ;
Bassler, Bonnie L. ;
Hughson, Frederick M. .
MOLECULAR CELL, 2011, 42 (02) :199-209
[12]   Anti-Quorum Sensing Potential of Crude Kigelia africana Fruit Extracts [J].
Chenia, Hafizah Y. .
SENSORS, 2013, 13 (03) :2802-2817
[13]   Inhibition of bacterial quorum sensing by vanilla extract [J].
Choo, J. H. ;
Rukayadi, Y. ;
Hwang, J. -K. .
LETTERS IN APPLIED MICROBIOLOGY, 2006, 42 (06) :637-641
[14]  
CLSI, 2021, Performance standards for antimicrobial susceptibility testing, M100, V31st
[15]   Calpurnia aurea (Aiton) Benth Extracts Reduce Quorum Sensing Controlled Virulence Factors in Pseudomonas aeruginosa [J].
Cosa, Sekelwa ;
Rakoma, Jostina R. ;
Yusuf, Abdullahi A. ;
Tshikalange, Thilivhali E. .
MOLECULES, 2020, 25 (10)
[16]   Exploring Common Culinary Herbs and Spices as Potential Anti-Quorum Sensing Agents [J].
Cosa, Sekelwa ;
Chaudhary, Sushil Kumar ;
Chen, Weiyang ;
Combrinck, Sandra ;
Viljoen, Alvaro .
NUTRIENTS, 2019, 11 (04)
[17]   Comparison of seven structurally related coumarins on the inhibition of Quorum sensing of Pseudomonas aeruginosa and Chromobacterium violaceum [J].
D'Almeida, R. E. ;
Molina, R. D. I. ;
Viola, C. M. ;
Luciardi, M. C. ;
Nieto Penalver, C. ;
Bardon, A. ;
Arena, M. E. .
BIOORGANIC CHEMISTRY, 2017, 73 :37-42
[18]   Bacterial biofilm development as a multicellular adaptation: antibiotic resistance and new therapeutic strategies [J].
de la Fuente-Nunez, Cesar ;
Reffuveille, Fany ;
Fernandez, Lucia ;
Hancock, Robert E. W. .
CURRENT OPINION IN MICROBIOLOGY, 2013, 16 (05) :580-589
[19]   Outer membrane permeability and antibiotic resistance [J].
Delcour, Anne H. .
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 2009, 1794 (05) :808-816
[20]   A sensitive and quick microplate method to determine the minimal inhibitory concentration of plant extracts for bacteria [J].
Eloff, JN .
PLANTA MEDICA, 1998, 64 (08) :711-713