Wheat Yellow Mosaic Virus NIb Interacting with Host Light Induced Protein (LIP) Facilitates Its Infection through Perturbing the Abscisic Acid Pathway in Wheat

被引：35

作者：

Zhang, Tianye ^{[1
,2
]}

Liu, Peng ^{[2
]}

Zhong, Kaili ^{[2
]}

Zhang, Fan ^{[2
]}

Xu, Miaoze ^{[2
]}

He, Long ^{[2
]}

Jin, Peng ^{[2
]}

Chen, Jianping ^{[1
,2
]}

Yang, Jian ^{[2
]}

机构：

[1] Zhejiang Agr & Forestry Univ, Sch Forestry & Biotechnol, Hangzhou 310021, Zhejiang, Peoples R China

[2] Ningbo Univ, Inst Plant Virol, State Key Lab Qual & Safety Agroprod, Ningbo 315211, Zhejiang, Peoples R China

来源：

BIOLOGY-BASEL | 2019年 / 8卷 / 04期

关键词：

wheat yellow mosaic virus; BIFC; ABA signaling pathway; DEPENDENT RNA-POLYMERASE; RESISTANCE; GENE; ACCUMULATION; EXPRESSION; MUTATION; COMPLEX; ENZYME; PLANTS;

D O I：

10.3390/biology8040080

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Positive-sense RNA viruses have a small genome with very limited coding capacity and are highly reliant on host factors to fulfill their infection. However, few host factors have been identified to participate in wheat yellow mosaic virus (WYMV) infection. Here, we demonstrate that wheat (Triticum aestivum) light-induced protein (TaLIP) interacts with the WYMV nuclear inclusion b protein (NIb). A bimolecular fluorescence complementation (BIFC) assay displayed that the subcellular distribution patterns of TaLIP were altered by NIb in Nicotiana benthamiana. Transcription of TaLIP was significantly decreased by WYMV infection and TaLIP-silencing wheat plants displayed more susceptibility to WYMV in comparison with the control plants, suggesting that knockdown of TaLIP impaired host resistance. Moreover, the transcription level of TaLIP was induced by exogenous abscisic acid (ABA) stimuli in wheat, while knockdown of TaLIP significantly repressed the expression of ABA-related genes such as wheat abscisic acid insensitive 5 (TaABI5), abscisic acid insensitive 8 (TaABI8), pyrabatin resistance 1-Llike (TaPYL1), and pyrabatin resistance 3-Llike (TaPYL3). Collectively, our results suggest that the interaction of NIb with TaLIP facilitated the virus infection possibly by disturbing the ABA signaling pathway in wheat.

引用

页数：15

共 41 条

[41] Virus-induced gene complementation reveals a transcription factor network in modulation of tomato fruit ripening [J].

Zhou, Tao ;

Zhang, Hang ;

Lai, Tongfei ;

Qin, Cheng ;

Shi, Nongnong ;

Wang, Huizhong ;

Jin, Mingfei ;

Zhong, Silin ;

Fan, Zaifeng ;

Liu, Yule ;

Wu, Zirong ;

Jackson, Stephen ;

Giovannoni, James J. ;

Rolin, Dominique ;

Gallusci, Philippe ;

Hong, Yiguo .

SCIENTIFIC REPORTS, 2012, 2

← 1 2 3 4 5 →