Sol-Gel-Derived Biohybrid Materials Incorporating Long-Chain DNA Aptamers

被引:18
作者
Carrasquilla, Carmen [1 ,2 ]
Kapteyn, Emily [1 ,2 ]
Li, Yingfu [1 ,2 ]
Brennan, John D. [1 ,2 ]
机构
[1] McMaster Univ, Biointerfaces Inst, 1280 Main St West, Hamilton, ON L8S 0A3, Canada
[2] McMaster Univ, Dept Biochem & Biomed Sci, 1280 Main St West, Hamilton, ON L8S 0A3, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
aptamers; hybrid materials; material screening; sol-gel processes; FRONTAL AFFINITY-CHROMATOGRAPHY; LIQUID-CHROMATOGRAPHY; MOLECULAR RECOGNITION; MASS-SPECTROMETRY; SILICA MATERIALS; PHASE ASSAYS; ENTRAPMENT; COLUMNS; THROMBIN; OPTIMIZATION;
D O I
10.1002/anie.201702859
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Sol-gel-derived bio/inorganic hybrid materials have been examined for diverse applications, including biosensing, affinity chromatography and drug discovery. However, such materials have mostly been restricted to the interaction between entrapped biorecognition elements and small molecules, owing to the requirement for nanometer-scale mesopores in the matrix to retain entrapped biorecognition elements. Herein, we report on a new class of macroporous bio/inorganic hybrids, engineered through a high-throughput materials screening approach, that entrap micron-sized concatemeric DNA aptamers. We demonstrate that the entrapment of these long-chain DNA aptamers allows their retention within the macropores of the silica material, so that aptamers can interact with high molecular weight targets such as proteins. Our approach overcomes the major limitation of previous sol-gel-derived biohybrid materials by enabling molecular recognition for targets beyond small molecules.
引用
收藏
页码:10686 / 10690
页数:5
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