Assessment of the reproducibility of oligoclonal IgM band detection for its application in daily clinical practice

被引：16

作者：

Espino, M. ^{[1
,15
]}

Abraira, V. ^{[2
]}

Arroyo, R. ^{[3
,15
]}

Bau, L. ^{[4
]}

Camara, C. ^{[5
]}

Campos-Ruiz, L. ^{[6
,15
]}

Casanova, B. ^{[7
,15
]}

Espejo, C. ^{[8
,15
]}

Fernandez, O. ^{[9
,15
]}

Garcia-Merino, A. ^{[6
,15
]}

Garcia-Sanchez, M. I. ^{[10
,15
]}

Gomez, M. ^{[18
]}

Gosis, A. ^{[15
,19
]}

Izquierdo, G. ^{[10
,15
]}

Meca, J. ^{[11
,15
]}

Montalban, X. ^{[8
,15
]}

Morandeira, F. ^{[17
]}

Olascoaga, J. ^{[12
,15
]}

Prada, A. ^{[15
,21
]}

Quintana, E. ^{[13
,15
]}

Ramio-Torrenta, Ll. ^{[13
,15
]}

Rodriguez-Antigueedad, A. ^{[14
,15
]}

Salgado, G. ^{[15
,20
]}

Santiago, J. L. ^{[3
,15
]}

Sarasola, E. ^{[14
,15
]}

Simo-Castello, M. ^{[7
,15
]}

Alvarez-Cermeno, J. C. ^{[15
,16
]}

Villar, L. M. ^{[1
,15
]}

机构：

[1] Hosp Univ Ramon y Cajal, IRYCIS, Dept Immunol, Madrid 28034, Spain

[2] Hosp Univ Ramon y Cajal, IRYCIS, Dept Biostat, Madrid 28034, Spain

[3] Hosp Clin San Carlos, Dept Neurol, Madrid 28040, Spain

[4] Hosp Univ Bellvitge, Dept Neurol, Barcelona, Spain

[5] Hosp San Pedro de Alcantara, Dept Immunol, Caceres 10003, Spain

[6] Hosp Univ Puerta de Hierro, Dept Neurol, Madrid 28222, Spain

[7] Hosp Univ La Fe, Dept Neurol, Valencia 46026, Spain

[8] Univ Autonoma Barcelona, Hosp Univ Vall dHebron, Vall dHebron Inst Recerca, Serv Neurol Neuroimmunol,Ctr Esclerosi Multiple C, Barcelona 08035, Spain

[9] Hosp Reg Univ, Dept Neurol, Malaga 29010, Spain

[10] Hosp Univ Virgen Macarena, Dept Neurol, Seville 41007, Spain

[11] Hosp Univ Virgen de la Arrixaca, Dept Neurol, Murcia 30120, Spain

[12] Hosp Univ Donostia, Dept Neurol, San Sebastian 20014, Spain

[13] Hosp Univ Dr Josep Trueta, Inst Invest Biomed Girona IDIBGI, Unitat Neuroimmunol & Esclerosi Multiple, Girona 17007, Spain

[14] Hosp Univ Basurto, Dept Neurol, Bilbao 48013, Spain

[15] Red Espanola Esclerosis Multiple, Madrid, Spain

[16] Hosp Univ Ramon y Cajal, IRYCIS, Dept Neurol, Madrid 28034, Spain

[17] Hosp Univ Bellvitge, Barcelona, Spain

[18] Hosp San Pedro de Alcantara, Dept Neurol, Caceres 10003, Spain

[19] Hosp Reg Univ, Malaga 29010, Spain

[20] Hosp Univ Virgen de la Arrixaca, Murcia 30120, Spain

[21] Hosp Univ Donostia, Dept Neurol, San Sebastian 20014, Spain

来源：

CLINICA CHIMICA ACTA | 2015年 / 438卷

关键词：

Immunoglobulin IgM; Oligoclonal bands; Multiple sclerosis; Biomarker; MULTIPLE-SCLEROSIS; CEREBROSPINAL-FLUID; BIOMARKERS; DISEASES; MS;

D O I：

10.1016/j.cca.2014.08.004

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Background: The presence of oligoclonal IgM bands (OCMB) in cerebrospinal fluid (CSF) is an unfavourable prognostic marker in multiple sclerosis. There is no commercial test to investigate OCMB status. However, a sensitive and specific isoelectrofocusing (IEF) and western blot method was described. We aimed to study the inter-centre reproducibility of this technique, a necessary condition for a reliable test to be incorporated into clinical practice. Methods: The presence of OCMB was analysed by IEF and western blot with prior reduction of pentameric IgM. We assayed the reproducibility of this test in a blinded multicentre study performed in 13 university hospitals. Paired-CSF and serum samples from 52 neurological patients were assayed at every centre. Results: Global analysis rendered a concordance of 89.8% with a kappa value of 0.71. Conclusion: These data indicate that OCMB detection by means of IEF and western blot with IgM reduction shows a good interlaboratory reproducibility and thus can be used in daily clinical setting. 2014 Elsevier B.V. All rights reserved.

引用

页码：67 / 69

页数：3

共 11 条

[1] Utility of oligoclonal IgG band detection for MS diagnosis in daily clinical practice [J].

Abraira, V. ;

Alvarez-Cermeno, J. C. ;

Arroyo, R. ;

Camara, C. ;

Casanova, B. ;

Cubillo, S. ;

de Andres, C. ;

Espejo, C. ;

Fernandez, O. ;

Ferrer, J. ;

Figueredo, M. A. ;

Garcia-Merino, A. ;

Garcia-Sanchez, M. I. ;

Garcia-Trujillo, J. A. ;

Gomez, M. ;

Gonzalez-Oria, C. ;

Gosis, A. ;

Izquierdo, G. ;

Jimenez, J. ;

Lopez-Trascasa, M. ;

Montalban, X. ;

Moreno, M. J. ;

Munoz, D. ;

Nunez, V. ;

Muriel, A. ;

Navarro, J. ;

Olascoaga, J. ;

Oreja-Guevara, C. ;

Prada, A. ;

Ramil, E. ;

Ramo-Tello, C. ;

Rodriguez, C. ;

Rodriguez, E. ;

Rodriguez-Frias, F. ;

Rodriguez-Antigueedad, A. ;

Rodriguez-Molina, J. J. ;

Ruiz, E. ;

Saiz, A. ;

Sarasola, E. ;

Simo, M. ;

Yaguee, J. ;

Villar, L. M. .

JOURNAL OF IMMUNOLOGICAL METHODS, 2011, 371 (1-2) :170-173

[2] Cerebrospinal fluid and plasma biomarkers in Alzheimer disease [J].

Blennow, Kaj ;

Hampel, Harald ;

Weiner, Michael ;

Zetterberg, Henrik .

NATURE REVIEWS NEUROLOGY, 2010, 6 (03) :131-144

[3] Cerebrospinal fluid oligoclonal IgM bands predict early conversion to clinically definite multiple sclerosis in patients with Clinically Isolated Syndrome [J].

Ferraro, Diana ;

Simone, Anna Maria ;

Bedin, Roberta ;

Galli, Veronica ;

Vitetta, Francesca ;

Federzoni, Lucia ;

D'Amico, Roberto ;

Merelli, Elisa ;

Nichelli, Paolo Frigio ;

Sola, Patrizia .

JOURNAL OF NEUROIMMUNOLOGY, 2013, 257 (1-2) :76-81

[4] Cancer biomarkers: selecting the right drug for the right patient [J].

Kelloff, Gary J. ;

Sigman, Caroline C. .

NATURE REVIEWS DRUG DISCOVERY, 2012, 11 (03) :201-214

[5] MEASUREMENT OF OBSERVER AGREEMENT FOR CATEGORICAL DATA [J].

LANDIS, JR ;

KOCH, GG .

BIOMETRICS, 1977, 33 (01) :159-174

[6] Recommendations for clinical use of data on neutralising antibodies to interferon-beta therapy in multiple sclerosis [J].

Polman, Chris H. ;

Bertolotto, Antonio ;

Deisenhammer, Florian ;

Giovannoni, Gavin ;

Hartung, Hans-Peter ;

Hemmer, Bernhard ;

Killestein, Joep ;

McFarland, Henry F. ;

Oger, Joel ;

Pachner, Andrew R. ;

Petkau, John ;

Reder, Anthony T. ;

Reingold, Stephen C. ;

Schellekens, Huub ;

Sorensen, Per Soelberg .

LANCET NEUROLOGY, 2010, 9 (07) :740-750

[7] Mechanistic biomarkers for clinical decision making in rheumatic diseases [J].

Robinson, William H. ;

Lindstrom, Tamsin M. ;

Cheung, Regina K. ;

Sokolove, Jeremy .

NATURE REVIEWS RHEUMATOLOGY, 2013, 9 (05) :267-276

[8] Lipid-specific immunoglobulin M in CSF predicts adverse long-term outcome in multiple sclerosis [J].

Thangarajh, M. ;

Gomez-Rial, J. ;

Hedstroem, A. K. ;

Hillert, J. ;

Alvarez-Cermeno, J. C. ;

Masterman, T. ;

Villar, L. M. .

MULTIPLE SCLEROSIS JOURNAL, 2008, 14 (09) :1208-1213

[9] Intrathecal IgM synthesis is a prognostic factor in multiple sclerosis [J].

Villar, LM ;

Masjuan, J ;

González-Porqué, P ;

Plaza, J ;

Sádaba, MC ;

Roldán, E ;

Bootello, A ;

Alvarez-Cermeño, JC .

ANNALS OF NEUROLOGY, 2003, 53 (02) :222-226

[10] Intrathecal IgM synthesis in neurologic diseases:: Relationship with disability in MS [J].