Recycling high-speed countercurrent chromatography for separation of taxol and cephalomannine

被引：34

作者：

Du, QZ ^{[1
]}

Ke, CQ

Ito, Y

机构：

[1] Chinese Acad Agr Sci, Tea Res Inst, Chinese Minist Agr, Key Lab Tea Chem Engn, Hangzhou 310008, Zhejiang, Peoples R China

[2] NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 1998年 / 21卷 / 1-2期

关键词：

D O I：

10.1080/10826079808001944

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A mixture of taxol and cephalomannine was subjected to recycling countercurrent chromatography (CCC) which allows the use of the same column for repetitive separation of the peak fractions to improve the peak resolution. When the total amount of 50 mg of the two components was recycled twice, peak resolution (R-s) increased from 0.7 to 1.27. The results also showed that R, value increases according to the formula: Rs-n = n(1/2)R(s-1) where n is the number of CCC runs, and Rs-1 and Rs-n, peak resolutions after the first and the nth run, respectively.

引用

页码：157 / 162

页数：6