Direct involvement of CD56 in cytokine-induced killer-mediated lysis of CD56+ hematopoietic target cells

被引:29
作者
Valgardsdottir, Rut [1 ]
Capitanio, Cristina [1 ]
Texido, Gemrna [2 ]
Pende, Daniela [3 ]
Cantoni, Claudia [4 ,5 ,6 ]
Pesenti, Enrico [2 ]
Rambaldi, Alessandro [1 ]
Golay, Josee [1 ]
Introna, Martino [1 ]
机构
[1] USS Ctr Cellular Therapy GLanzani, USC Hematol & Bone Marrow Transplantat Unit, I-24128 Bergamo, Italy
[2] Nerviano Med Sci, Nerviano, Italy
[3] IRCCS AOU San Martino, IST, Genoa, Italy
[4] Ist Giannina Gaslini, I-16148 Genoa, Italy
[5] Univ Genoa, Dipartimento Med Sperimentale, Genoa, Italy
[6] Univ Genoa, Ctr Eccellenza Ric Biomed, Genoa, Italy
关键词
ADHESION MOLECULE; NK CELLS; CYTOTOXICITY; EXPRESSION; ANTIGEN; IDENTIFICATION; LYMPHOCYTES; ISOFORMS; CORD;
D O I
10.1016/j.exphem.2014.08.005
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Cytokine-induced killer (CIK) cells are in-vitro-expanded T lymphocytes that represent a heterogeneous population. A large majority of CIK cells are CD3(+)CD56(+), and this population has been shown to confer a cytotoxic effect against tumor targets. The scope of this work was to study whether CD56 has a direct role in CIK-mediated cytotoxicity. Blocking of CD56 with the anti-CD56 monoclonal antibody GPR165 significantly reduced CIK-mediated lysis of three CD56(+) hematopoietic tumor cell lines (AML-NS8, NB4, and KCL22), whereas no effect was observed on three CD56(-) hematopoietic tumor cell lines (K562, REH, and MOLT-4). Knockdown of CD56(+) in CIK cells by short interfering RNA made the cells less cytotoxic against a CD56+ target, and knockdown of CD56 in target cells with lentiviral short hairpin RNA significantly altered their susceptibility to CIK-mediated lysis. Our data suggest that homophilic interaction between CD56 molecules may occur in tumor-cell recognition, leading to CIK-mediated cell death. Copyright (C) 2014 ISEH - International Society for Experimental Hematology. Published by Elsevier Inc.
引用
收藏
页码:1013 / 1021
页数:9
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