Visualization of a Mammalian Mitochondrion by Coherent X-ray Diffractive Imaging

被引：15

作者：

Kim, Yoonhee ^{[1
,2
]}

Kim, Chan ^{[1
,2
,3
]}

Kwon, Ou Young ^{[1
,2
]}

Nam, Daewoong ^{[4
]}

Kim, Sang Soo ^{[5
]}

Park, Jae Hyun ^{[5
]}

Kim, Sunam ^{[5
]}

Gallagher-Jones, Marcus ^{[6
,7
,8
]}

Kohmura, Yoshiki ^{[6
]}

Ishikawa, Tetsuya ^{[6
]}

Song, Changyong ^{[4
,6
]}

Tae, Giyoong ^{[1
,2
]}

Noh, Do Young ^{[1
,2
]}

机构：

[1] Gwangju Inst Sci & Technol, Dept Phys & Photon Sci, Gwangju 61005, South Korea

[2] Gwangju Inst Sci & Technol, Sch Mat Sci & Engn, Gwangju 61005, South Korea

[3] European XFEL, D-22869 Schenefeld, Germany

[4] Pohang Univ Sci & Technol, Dept Phys, Pohang 37673, South Korea

[5] Pohang Univ Sci & Technol, Pohang Accelerator Lab, Pohang 37673, South Korea

[6] RIKEN, SPring Ctr 8, Kouto 1-1-1, Tokyo, Hyogo 6795148, Japan

[7] Univ Calif Los Angeles, Dept Phys & Astron, Los Angeles, CA 90095 USA

[8] Univ Calif Los Angeles, Calif NanoSyst Inst, Los Angeles, CA 90095 USA

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

基金：

新加坡国家研究基金会;

关键词：

ELECTRON TOMOGRAPHY; RESOLUTION; MICROSCOPY; ENHANCEMENT; CRISTAE; CELLS; WHOLE;

D O I：

10.1038/s41598-017-01833-x

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We report a three dimensional (3D) quantitative visualization of a mammalian mitochondrion by coherent x-ray diffractive imaging (CXDI) using synchrotron radiation. The internal structures of a mitochondrion from a mouse embryonic fibroblast cell line (NIH3T3) were visualized by tomographic imaging at approximately 60 nm resolution without the need for sectioning or staining. The overall structure consisted of a high electron density region, composed of the outer and inner membranes and the cristae cluster, which enclosed the lower density mitochondrial matrix. The average mass density of the mitochondrion was about 1.36 g/cm(3). Sectioned images of the cristae reveal that they have neither a baffle nor septa shape but were instead irregular. In addition, a high resolution, about 14 nm, 2D projection image was captured of a similar mitochondrion with the aid of strongly scattering Au reference objects. Obtaining 3D images at this improved resolution will allow CXDI to be an effective and nondestructive method for investigating the innate structure of mitochondria and other important life supporting organelles.

引用

页数：9

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