Breast cancer upregulating genes in stromal cells by LINE-1 hypermethylation and micrometastatic detection

被引：18

作者：

Puttipanyalears, Charoenchai ^{[1
,2
]}

Kitkumthorn, Nakarin ^{[3
]}

Buranapraditkun, Supranee ^{[4
]}

Keelawat, Somboon ^{[5
]}

Mutirangura, Apiwat ^{[2
]}

机构：

[1] Chulalongkorn Univ, Fac Grad Sch, Interdept Program BioMed Sci, Bangkok, Thailand

[2] Chulalongkorn Univ, Fac Med, Dept Anat, Ctr Excellence Mol Genet Canc & Human Dis, Bangkok 10330, Thailand

[3] Mahidol Univ, Fac Dent, Dept Oral Biol, Bangkok 10700, Thailand

[4] Chulalongkorn Univ, Fac Med, Dept Med, Div Allergy & Clin Immunol, Bangkok 10330, Thailand

[5] Chulalongkorn Univ, Fac Med, Dept Pathol, Bangkok 10330, Thailand

来源：

EPIGENOMICS | 2016年 / 8卷 / 04期

关键词：

breast cancer; LINE-1; hypermethylation; tumor supporting tissue; METHYLATION PATTERNS; TGF-BETA; FIBROBLASTS; CARCINOMA; PROLIFERATION; SIGNATURES;

D O I：

10.2217/epi-2015-0007

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Changes in the methylation level of genes containing LINE-1 alter host gene regulation. Aim: This study demonstrates that paracrine signaling of breast cancer influences the epigenetic regulation of stromal cells. Methods: We proved in vitro and in vivo breast cancer promoted LINE-1 methylation exists exclusively in female stromal cells. Results: Genes containing LINE-1 of breast cancer stromal cells were upregulated. Furthermore, one of the genes, MUC-1, was demonstrated to have expression in plasma cells from the lymph nodes of patients with lymph node metastasis or micrometastasis. Conclusion: Breast cancer sends a paracrine signal to stroma cells causing LINE-1 epigenetic regulation. Moreover, the regulated genes in stroma cells are potential biomarkers for detecting breast cancer micrometastasis.

引用

页码：475 / 486

页数：12

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