Diazonium-based impedimetric aptasensor for the rapid label-free detection of Salmonella typhimurium in food sample

被引:116
作者
Bagheryan, Zahra [1 ,2 ]
Raoof, Jahan-Bakhsh [2 ]
Golabi, Mohsen [1 ]
Turner, Anthony P. F. [1 ]
Beni, Valerio [1 ,3 ]
机构
[1] Linkoping Univ, Dept Phys Chem & Biol IFM, Biosensors & Bioelect Ctr, S-58183 Linkoping, Sweden
[2] Univ Mazandaran, Fac Chem, Dept Analyt Chem, Eletroanalyt Chem Res Lab, Babol Sar, Iran
[3] Acreo Swedish ICT AB, Box 787, SE-60117 Norrkoping, Sweden
关键词
Diazonium grafting; Aptamer; S; typhimurium; Label-free detection; Electrochemical impedance spectroscopy; Food analysis; UNITED-STATES; ANTIBODY IMMOBILIZATION; TETRATHIONATE BROTH; PATHOGEN DETECTION; ELECTRON-TRANSFER; CARBON SURFACE; DNA APTAMERS; GOLD; LAYERS; SENSOR;
D O I
10.1016/j.bios.2016.02.024
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Fast and accurate detection of microorganisms is of key importance in clinical analysis and in food and water quality monitoring. Salmonella typhimurium is responsible for about a third of all cases of food borne diseases and consequently, its fast detection is of great importance for ensuring the safety of foodstuffs. We report the development of a label-free impedimetric aptamer-based biosensor for S. typhimurium detection. The aptamer biosensor was fabricated by grafting a diazonium-supporting layer onto screen printed carbon electrodes (SPEs), via electrochemical or chemical approaches, followed by chemical immobilisation of aminated-aptamer. FTIR-ATR, contact angle and electrochemical measurements were used to monitor the fabrication process. Results showed that electrochemical immobilisation of the diazonium-grafting layer allowed the formation of a denser aptamer layer, which resulted in higher sensitivity. The developed aptamer-biosensor responded linearly, on a logarithm scale, over the concentration range 1 x 10(1) to 1 x 10(8) CFU mL(-1), with a limit of quantification (LOQ) of 1 x 10(1) CFU mL(-1) and a limit of detection (LOD) of 6 CFU mL(-1). Selectivity studies showed that the aptamer biosensor could discriminate S. typhimurium from 6 other model bacteria strains. Finally, recovery studies demonstrated its suitability for the detection of S. typhimurium in spiked (1 x 10(2), 1 x 10(4) and 1 x 10(6) CFU mL(-1)) apple juice samples. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码:566 / 573
页数:8
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