Intestinal CD8αα and CD8αβ intraepithelial lymphocytes are thymus derived and exhibit subtle differences in TCRβ repertoires

被引:22
作者
Imhof, BA
Dunon, D
Courtois, D
Luhtala, M
Vainio, O
机构
[1] Univ Geneva, Ctr Med, Dept Pathol, CH-1211 Geneva, Switzerland
[2] Univ Paris 06, CNRS, UMR 7622, Paris, France
[3] Univ Turku, Dept Med Microbiol, Turku, Finland
关键词
D O I
10.4049/jimmunol.165.12.6716
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Intraepithelial lymphocytes (IEL) of the small intestine are anatomically positioned to be in the first line of cellular defense against enteric pathogens. Therefore, determining the origin of these cells has important implications for the mechanisms of T cell maturation and repertoire selection. Recent evidence suggests that murine CD8 alpha alpha intestinal IELs (iIELs) can mature and undergo selection in the absence of a thymus, We analyzed IEL origin by cell transfer, using two congenic chicken strains. Embryonic day 14 and adult thymocytes did not contain any detectable CD8 alpha alpha T cells. However, when TCR+ thymocytes were injected into congenic animals, they migrated to the gut and developed into CD8 alpha alpha iIELs, while TCR- T cell progenitors did not. The TCR V beta1 repertoire of CD8 alpha alpha (+) TCR V beta1(+) iIELs contained only part of the TCR V beta1 repertoire of total iIELs, and it exhibited no new members compared with CD8(+) T cells in the thymus, This indicated that these T cells emigrated from the thymus at an early stage in their developmental process. In conclusion, we show that while CD8 alpha alpha iIELs originate in the thymus, T cells acquire the expression of CD8 alpha alpha homodimers in the gut microenvironment.
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页码:6716 / 6722
页数:7
相关论文
共 45 条
[1]   Identical T cell clones are located within the mouse gut epithelium and lamina propria and circulate in the thoracic duct lymph [J].
Arstila, T ;
Arstila, TP ;
Calbo, S ;
Selz, F ;
Malassis-Seris, M ;
Vassalli, P ;
Kourilsky, P ;
Guy-Grand, D .
JOURNAL OF EXPERIMENTAL MEDICINE, 2000, 191 (05) :823-834
[2]   SELECTION OF INTESTINAL INTRAEPITHELIAL LYMPHOCYTE T-CELL RECEPTORS - EVIDENCE FOR A DYNAMIC TISSUE-SPECIFIC PROCESS [J].
BADINER, G ;
GOODMAN, TG ;
LEFRANCOIS, L .
INTERNATIONAL IMMUNOLOGY, 1993, 5 (02) :223-226
[3]   VARIABILITY ANALYSIS OF THE HUMAN AND MOUSE T-CELL RECEPTOR BETA-CHAINS [J].
BOUGUELERET, L ;
CLAVERIE, JM .
IMMUNOGENETICS, 1987, 26 (4-5) :304-308
[4]  
BUCY RP, 1990, J IMMUNOL, V144, P1161
[5]  
BUCY RP, 1988, J IMMUNOL, V141, P2200
[6]  
CHAR D, 1990, J IMMUNOL, V145, P3547
[7]  
Chen CH, 1996, CURR TOP MICROBIOL, V212, P37
[8]   CHARACTERIZATION AND FUNCTIONAL-PROPERTIES OF A NOVEL MONOCLONAL-ANTIBODY WHICH IDENTIFIES A T-CELL RECEPTOR IN CHICKENS [J].
CIHAK, J ;
ZIEGLERHEITBROCK, HWL ;
TRAINER, H ;
SCHRANNER, I ;
MERKENSCHLAGER, M ;
LOSCH, U .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1988, 18 (04) :533-537
[9]   ANALYSIS OF THE 1ST 2 WAVES OF THYMUS HOMING STEM-CELLS AND THEIR T-CELL PROGENY IN CHICK-QUAIL CHIMERAS [J].
COLTEY, M ;
BUCY, RP ;
CHEN, CH ;
CIHAK, J ;
LOSCH, U ;
CHAR, D ;
LEDOUARIN, NM ;
COOPER, MD .
JOURNAL OF EXPERIMENTAL MEDICINE, 1989, 170 (02) :543-557
[10]   EVIDENCE FOR A CYCLIC RENEWAL OF LYMPHOCYTE PRECURSOR CELLS IN THE EMBRYONIC CHICK THYMUS [J].
COLTEY, M ;
JOTEREAU, FV ;
LEDOUARIN, NM .
CELL DIFFERENTIATION, 1987, 22 (01) :71-82