Effects of calmodulin on DNA-binding activity of heat shock transcription factor in vitro

被引：4
|
作者：
Li, B
Liu, HT
Mu, RL
Sun, DY [1 ]
Zhou, RG
机构：
[1] Hebei Normal Univ, Inst Mol Cell Biol, Shijiazhuang 050016, Peoples R China
[2] Hebei Acad Agr & Forestry Sci, Inst Agrophys Physiol & Biochem, Shijiazhuang 050051, Peoples R China
来源：
CHINESE SCIENCE BULLETIN | 2003年 / 48卷 / 03期
关键词：
calmodulin; heat shock transcription factor; DNA-binding activity; maize; wheat; tomato;
D O I：
10.1360/03tb9052
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The DNA-binding activity of heat shock transcription factor (HSF) was induced by heat shock (HS) of a whole cell extract. Addition of antiserum, specific to CaM, to a whole cell extract reduced bind of the HSF to the heat shock element (HSE) with maize, and the re-addition of CaM to the sample restored the activity of the HSF for binding to HSE. In addition, DNA-binding activity of the HSF was also induced by directly adding CaM to a whole cell extract at non-HS temperature with maize. Similar results were obtained with wheat and tomato. Our observations provide the first example of the involvement of CaM in regulation of the DNA-binding activity of the HSF.
引用
收藏
页码：255 / 258
页数：4
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