Microfluidic model for in vitro acute Toxoplasma gondii infection and transendothelial migration

被引：5
|
作者：
Kim, Hyunho [1 ,2 ]
Hong, Sung-Hee [3 ]
Jeong, Hyo Eun [1 ]
Han, Sewoon [4 ]
Ahn, Jinchul [1 ]
Kim, Jin-A. [1 ]
Yang, Ji-Hun [5 ]
Oh, Hyun Jeong [1 ]
Chung, Seok [1 ,6 ]
Lee, Sang-Eun [3 ]
机构：
[1] Korea Univ, Sch Mech Engn, Seoul, South Korea
[2] Massachusetts Gen Hosp, Res Inst, Ctr Syst Biol, Boston, MA 02114 USA
[3] Korea Dis Control & Prevent Agcy, Div Vectors & Parasit Dis, Cheongju, South Korea
[4] CellFE, Alameda, CA USA
[5] Next & Bio, Seoul, South Korea
[6] Korea Univ, KU KIST Grad Sch Converging Sci & Technol, Seoul, South Korea
来源：
SCIENTIFIC REPORTS | 2022年 / 12卷 / 01期
基金：
新加坡国家研究基金会;
关键词：
MURINE MACROPHAGES; ENDOTHELIAL-CELLS; TACHYZOITES; INHIBITION; MODULATION; CULTURE; MYOSIN; EGRESS; GROWTH; GAMMA;
D O I：
10.1038/s41598-022-15305-4
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The protozoan parasite Toxoplasma gondii (T. gondii) causes one of the most common human zoonotic diseases and infects approximately one-third of the global population. T. gondii infects nearly every cell type and causes severe symptoms in susceptible populations. In previous laboratory animal studies, T. gondii movement and transmission were not analyzed in real time. In a three-dimensional (3D) microfluidic assay, we successfully supported the complex lytic cycle of T. gondii in situ by generating a stable microvasculature. The physiology of the T. gondii-infected microvasculature was monitored in order to investigate the growth, paracellular and transcellular migration, and transmission of T. gondii, as well as the efficacy of T. gondii drugs.
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页数：9
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