Target-induced selection of ligands from a dynamic combinatorial library of mono- and bi-conjugated oligonucleotides

被引：15
作者：
Bugaut, A
Bathany, K
Schmitter, JM
Rayner, B
机构：
[1] Univ Bordeaux 2, INSERM, U386, F-33076 Bordeaux, France
[2] IECB, F-33607 Pessac, France
来源：
TETRAHEDRON LETTERS | 2005年 / 46卷 / 04期
关键词：
dynamic combinatorial chemistry; conjugated oligonucleotides; duplex stabilization;
D O I：
10.1016/j.tetlet.2004.11.110
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
A dynamic library of 15 mono- and bi-conjugated oligonucleotides was generated from a pool of three aldehydes and an oligonucleotide bearing two reactive amino groups. Addition of complementary target to the equilibrating mixture of imines resulted in selective amplification of one conjugate. UV-melting experiments confirmed that it was the best ligand among those that were tested. This study emphasizes that dynamic combinatorial chemistry can be used to simultaneously identify the type and the location of appended residues for stabilizing oligonucleotide complexes. (C) 2004 Elsevier Ltd. All rights reserved.
引用
收藏
页码：687 / 690
页数：4
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