Novel integrated and portable endotoxin detection system based on an electrochemical biosensor

被引：20

作者：

Zuzuarregui, Ana ^{[1
,2
,3
]}

Souto, David ^{[1
,2
,3
]}

Perez-Lorenzo, Eva ^{[1
,2
,3
]}

Arizti, Fernando ^{[1
,2
]}

Sanchez-Gomez, Susana ^{[4
]}

Martinez de Tejada, Guillermo ^{[4
]}

Brandenburg, Klaus ^{[5
]}

Arana, Sergio ^{[1
,2
,3
]}

Mujika, Maite ^{[1
,2
,3
]}

机构：

[1] Univ Navarra, CEIT, San Sebastian 20018, Spain

[2] Univ Navarra, Tecnun, San Sebastian 20018, Spain

[3] CIC Microgune, Arrasate Mondragon 20500, Spain

[4] Univ Navarra, Dept Microbiol & Parasitol, E-31008 Irunlarrea, Spain

[5] Leibniz Ctr Med & Biosci, Res Ctr Borstel, Div Biophys, Borstel, Germany

来源：

ANALYST | 2015年 / 140卷 / 02期

关键词：

POLYCRYSTALLINE GOLD ELECTRODES; SELF-ASSEMBLED MONOLAYERS; IMMOBILIZATION; PRETREATMENT; PEPTIDES; PROTEIN;

D O I：

10.1039/c4an01324g

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This paper describes the design, implementation and validation of a sensitive and integral technology solution for endotoxin detection. The unified and portable platform is based on the electrochemical detection of endotoxins using a synthetic peptide immobilized on a thin-film biosensor. The work covers the fabrication of an optimized sensor, the biofunctionalization protocol and the design and implementation of the measuring and signalling elements (a microfluidic chamber and a portable potentiostat-galvanostat), framed ad hoc for this specific application. The use of thin-film technologies to fabricate the biosensing device and the application of simple immobilization and detection methods enable a rapid, easy and sensitive technique for in situ and real time LPS detection.

引用

页码：654 / 660

页数：7

共 40 条

[1]

[Anonymous], 2005, EUROPEAN PHARMACOPOE, P161

[2]

Anorga L., 2011, Oficina Espanola de Patentes y Marcas - Espana, Patent No. [WO 2011/054982, G01N27/407D2, 2011054982]

[3] Comparative analysis of QCM and SPR techniques for the optimization of immobilization sequences [J].

Ansorena, P. ;

Zuzuarregui, A. ;

Perez-Lorenzo, E. ;

Mujika, M. ;

Arana, S. .

SENSORS AND ACTUATORS B-CHEMICAL, 2011, 155 (02) :667-672

[4] Gold immuno-functionalisation via self-assembled monolayers:: Study of critical parameters and comparative performance for protein and bacteria detection [J].

Baldrich, Eva ;

Laczka, Olivier ;

Javier Del Campo, Fco. ;

Xavier Munoz, Francesc .

JOURNAL OF IMMUNOLOGICAL METHODS, 2008, 336 (02) :203-212

[5]

Bard A. J., 2010, ELECTROCHEMICAL METH, V677

[6]

Blondelle S., 2008, World Intellectual Property Organization, Patent No. [WO 2008/7006125 A1, 20087006125]

[7] Realization and characterization of porous gold for increased protein coverage on acoustic sensors [J].

Bonroy, K ;

Friedt, JM ;

Frederix, F ;

Laureyn, W ;

Langerock, S ;

Campitelli, A ;

Sára, M ;

Borghs, G ;

Goddeeris, B ;

Declerck, P .

ANALYTICAL CHEMISTRY, 2004, 76 (15) :4299-4306

[8]

Brandenburg K, 2009, Patent, Patent No. [WO2009124721A1, 2009124721]

[9] Polycrystalline gold electrodes: A comparative study of pretreatment procedures used for cleaning and thiol self-assembly monolayer formation [J].

Carvalhal, RT ;

Freire, RS ;

Kubota, LT .

ELECTROANALYSIS, 2005, 17 (14) :1251-1259

[10] The immunopathogenesis of sepsis [J].

Cohen, J .

NATURE, 2002, 420 (6917) :885-891

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