Role of transamination in the mobilization of respiratory substrates in germinating seeds of castor oil plants

被引：4
|
作者：
Popov, V. N. [1 ]
Eprintsev, A. T.
Fedorin, D. N.
Fomenko, O. Yu.
Igamberdiev, A. U.
机构：
[1] Voronezh State Univ, Voronezh 394693, Russia
[2] Univ Manitoba, Dept Plant Sci, Winnipeg, MB R3T 2N2, Canada
来源：
APPLIED BIOCHEMISTRY AND MICROBIOLOGY | 2007年 / 43卷 / 03期
关键词：
D O I：
10.1134/S0003683807030179
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The metabolism of 1,4-C-14-succinate and 2,3-C-14-succinate and the activity of succinic semialdehyde dehydrogenase (EC 1.2.1.16) were studied in germinating seeds of castor oil plants (Ricinus communis L.). Succinate metabolism involved succinate dehydrogenase and was sensitive to metabolites of the gamma-aminobutyric acid shunt. Considerable accumulation of the label in amino acids reflected the progression of transamination reactions. Succinic semialdehyde dehydrogenase was purified from the endosperm of castor oil plants. Kinetic characteristics of the enzyme were evaluated. Our study indicates that the mobilization of respiratory substrates during germination of castor oil plants is related to active transamination of ketoacids in the Krebs cycle and involves the gamma-aminobutyric acid shunt.
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页码：341 / 346
页数：6
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