DHA Upregulates FADS2 Expression in Primary Cortical Astrocytes Exposed to Vitamin A

被引:9
作者
Dziedzic, B. [1 ]
Bewicz-Binkowska, D. [1 ]
Zgorzynska, E. [1 ]
Stulczewski, D. [1 ]
Wieteska, L. [2 ]
Kaza, B. [3 ]
Walczewska, A. [1 ]
机构
[1] Med Univ Lodz, Dept Cell To Cell Commun, Mazowiecka 6-8, PL-92415 Lodz, Poland
[2] Med Univ Lodz, Dept Med Biochem, Lodz, Poland
[3] Nencki Inst Expt Biol, Neurobiol Ctr, Lab Mol Neurobiol, Warsaw, Poland
关键词
FADS2; Vitamin A; Docosahexaenoic acid; alpha-linolenic acid; Palmitic acid; Astrocytes; POLYUNSATURATED FATTY-ACIDS; ELEMENT-BINDING PROTEIN-1; ACTIVATED RECEPTOR-ALPHA; DELTA-6 DESATURASE GENE; DOCOSAHEXAENOIC ACID; BRAIN; GAMMA; TRANSCRIPTION; LOCALIZATION; SUPPRESSION;
D O I
10.33549/physiolres.933708
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
The fads2 gene encoding Delta 6-desaturase, the rate-limiting enzyme of the LCPUFA biosynthesis is expressed in astrocytes. Dietary fatty acids, which cross the blood-brain barrier, may regulate the transcription of lipogenic enzymes through activation of transcription factors such as peroxisome proliferator-activated receptors (PPARs). The PPARs form the transcription complex with retinoid X receptors (RXRs) that are activated by 9-cis retinoic acid, a metabolite of vitamin A (VA). The study examines whether challenge of astrocytes with VA, prior 24-h treatment with palmitic acid (PA), alpha-linolenic acid (ALA) or docosahexaenoic acid (DHA) has the effect on the FADS2 expression. RT-qPCR showed that in astrocytes not challenged with VA, PA increased fads2 gene expression and DHA decreased it. However, in VA-primed astrocytes, PA doubled the FADS2 mRNA levels, while DHA increased fads2 gene expression, oppositely to non-primed cells. Furthermore, similar changes were seen in VA-primed astrocytes with regard to Delta 6-desaturase protein levels following PA and DHA treatment. ALA did not have any effect on the FADS2 mRNA and protein levels in either VA-primed or non-primed astrocytes. These findings indicate that in the presence of vitamin A, DHA upregulates fads2 gene expression in astrocytes.
引用
收藏
页码:663 / 668
页数:6
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