Specific role for protein kinase Cα in the constitutive and regulated secretion of amyloid precursor protein in human skin fibroblasts

被引：52

作者：

Benussi, L ^{[1
]}

Govoni, S ^{[1
]}

Gasparini, L ^{[1
]}

Binetti, G ^{[1
]}

Trabucchi, M ^{[1
]}

Bianchetti, A ^{[1
]}

Racchi, M ^{[1
]}

机构：

[1] Ctr San Giovanni di Dio Fatebenefratelli, IRCCS, Alzheimers Dis Unit, Cellular & Mol Neurobiol Lab, I-25123 Brescia, Italy

来源：

NEUROSCIENCE LETTERS | 1998年 / 240卷 / 02期

关键词：

Alzheimer's disease; amyloid precursor protein; human skin fibroblasts; protein kinase C; indolocarbazole Go-6976; phorbol esters; staurosporine;

D O I：

10.1016/S0304-3940(97)00894-X

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Reduced levels of protein kinase C alpha (PKC alpha) seems to be related to an altered amyloid precursor protein (APP) secretion in fibroblasts from Alzheimer's disease (AD) patients. In this report we used a specific inhibitor of PKC alpha (Go-6976), to investigate the role of PKC alpha in the basal and phorbol esters regulated secretion of soluble APP (sAPP) in human fibroblasts derived from healthy aged volunteers. Treatment with Go-6976 alone reduced basal secretion by a maximum of 39%, compared to untreated cells, suggesting the partial dependence of constitutive APP secretory pathway on PKC alpha enzyme. Moreover Go-6976 treatment completely abolished the effect of phorbol-esters mediated PKC stimulation on sAPP release, suggesting that PKC alpha is the only PKC isoform involved in controlling the secretion of sAPP in human fibroblasts. (C) 1998 Elsevier Science Ireland Ltd.

引用

页码：97 / 101

页数：5

共 22 条

[1] Defective phorbol ester-stimulated secretion of beta-amyloid precursor protein from Alzheimer's disease fibroblasts [J].