Osteoinductive 3D printed scaffold healed 5 cm segmental bone defects in the ovine metatarsus

被引：19

作者：

Yang, Yunzhi Peter ^{[1
,2
,3
]}

Labus, Kevin M. ^{[4
,5
]}

Gadomski, Benjamin C. ^{[4
,5
]}

Bruyas, Arnaud ^{[1
]}

Easley, Jeremiah ^{[6
]}

Nelson, Brad ^{[6
]}

Palmer, Ross H. ^{[6
]}

McGilvray, Kirk ^{[4
,5
]}

Regan, Daniel ^{[7
]}

Puttlitz, Christian M. ^{[4
,5
]}

Stahl, Alexander ^{[1
,8
]}

Lui, Elaine ^{[1
]}

Li, Jiannan ^{[1
]}

Moeinzadeh, Seyedsina ^{[1
]}

Kim, Sungwoo ^{[1
]}

Maloney, William ^{[1
]}

Gardner, Michael J. ^{[1
]}

机构：

[1] Stanford Univ, Sch Med, Dept Orthoped Surg, 240 Pasteur Dr,BMI 258, Stanford, CA 94304 USA

[2] Stanford Univ, Dept Mat Sci & Engn, Stanford, CA 94304 USA

[3] Stanford Univ, Dept Bioengn, Stanford, CA 94304 USA

[4] Colorado State Univ, Dept Mech Engn, Ft Collins, CO 80523 USA

[5] Colorado State Univ, Sch Biomed Engn, Ft Collins, CO 80523 USA

[6] Colorado State Univ, Dept Clin Sci, Ft Collins, CO 80523 USA

[7] Colorado State Univ, Dept Microbiol Immunol & Pathol, Ft Collins, CO 80523 USA

[8] Stanford Univ, Dept Chem, Stanford, CA 94305 USA

来源：

SCIENTIFIC REPORTS | 2021年 / 11卷 / 01期

关键词：

D O I：

10.1038/s41598-021-86210-5

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Autologous bone grafts are considered the gold standard grafting material for the treatment of nonunion, but in very large bone defects, traditional autograft alone is insufficient to induce repair. Recombinant human bone morphogenetic protein 2 (rhBMP-2) can stimulate bone regeneration and enhance the healing efficacy of bone grafts. The delivery of rhBMP-2 may even enable engineered synthetic scaffolds to be used in place of autologous bone grafts for the treatment of critical size defects, eliminating risks associated with autologous tissue harvest. We here demonstrate that an osteoinductive scaffold, fabricated by combining a 3D printed rigid polymer/ceramic composite scaffold with an rhBMP-2-eluting collagen sponge can treat extremely large-scale segmental defects in a pilot feasibility study using a new sheep metatarsus fracture model stabilized with an intramedullary nail. Bone regeneration after 24 weeks was evaluated by micro-computed tomography, mechanical testing, and histological characterization. Load-bearing cortical bridging was achieved in all animals, with increased bone volume observed in sheep that received osteoinductive scaffolds compared to sheep that received an rhBMP-2-eluting collagen sponge alone.

引用

页数：12