Molecular Dynamics Simulations of Competitive Protein Adsorption onto Chromatographic Media

被引：0
作者：
Liang, Juan [1 ]
Fieg, Georg [1 ]
Jakobtorweihen, Sven [2 ]
机构：
[1] Hamburg Univ Technol, Inst Proc & Plant Engn, D-21073 Hamburg, Germany
[2] Hamburg Univ Technol, Inst Thermal Separat Proc, D-21073 Hamburg, Germany
来源：
24TH EUROPEAN SYMPOSIUM ON COMPUTER AIDED PROCESS ENGINEERING, PTS A AND B | 2014年 / 33卷
关键词：
serum albumin; hemoglobin; ion-exchange adsorption; MD simulation; binary components; FORCE-FIELD; EXCHANGE;
D O I：
暂无
中图分类号：
TP39 [计算机的应用];
学科分类号：
081203 ; 0835 ;
摘要：
To get a further understanding of protein adsorption onto ion-exchange chromatographic media, the binary adsorption of serum albumin and hemoglobin onto Q Sepharose FF was investigated with molecular dynamics (MD) simulations. Considering the adsorption sequence, both simultaneous and sequential adsorption has been simulated. The protein underwent a competing adsorption. The results show that serum albumin is preferentially adsorbed. A multi-layer adsorption was observed in the sequential binary simulations. Hence, this study provides a detailed description of protein adsorption on a molecular scale.
引用
收藏
页码：937 / 942
页数：6
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